{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T01:36:25Z","timestamp":1745372185042},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":5535,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1989,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated ultrastructural cytochemical properties of elastic elements in <jats:italic>Alligator<\/jats:italic> periodontal ligaments decalcified with EDTA and stained with 1) the tannic acid\u2010uranyl acetate (TA\u2010UA) method for elastin in combination with elastase digestion; 2) the high iron diamine\u2010thiocarbohydrazide\u2010silver proteinate (HID\u2010TCH\u2010SP) method with prior treatment of specimens with either monopersulphate or cupric\u2010sulphite reagent for the localization of disulphide\u2010 and\/or sulphydryl\u2010containing material (i.e., oxytalan fibers); and 3) HID\u2010TCH\u2010SP alone for sulphated complex carbohydrates. Many microfibrils accumulated to form either large or small bundles. Large bundles having a diameter of 2.50 \u00b1 1.10 \u03bcm (mean \u00b1 SD; n = 50) each showed an apico\u2010occlusal distribution, although small bundles measuring 0.63 \u00b1 0.13 \u03bcm (mean \u00b1 SD; n = 50) in diameter each were exclusively localized in interstitial areas rich in vessels and nerves. The former bundles always lacked TA\u2010UA reactivity and represented oxytalan fibers; the latter bundles frequently contained TA\u2010UA\u2010reactive elastase digestable components and were similar in appearance to immature elastic fibers or elaunin fibers. HID\u2010TCH\u2010SP after oxidation strongly stained both the oxytalan and elastic fiber microfibrils but stained the amorphous elastin very weakly or not all. In nonoxidized specimens, there was no definite HID\u2010TCH\u2010SP staining of microfibrils and the amorphous elastin, although adjacent matrix proteoglycans stained consistently. These results indicate that although there is a marked difference in the distribution and size of oxytalan and elastic fibers in <jats:italic>Alligator<\/jats:italic> periodontal ligaments, their associated microfibrils lack stainable sulphate groups but are enriched with disulphide and\/or sulphydryl groups, as has been described in mammals.<\/jats:p>","DOI":"10.1002\/ar.1092250404","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T20:59:48Z","timestamp":1109451588000},"page":"279-287","source":"Crossref","is-referenced-by-count":6,"title":["Differential distribution and ultrastructural staining of oxytalan and elastic fibers in the periodontal ligament of <i>Alligator mississippiensis<\/i>"],"prefix":"10.1002","volume":"225","author":[{"given":"Minoru","family":"Takagi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshitada","family":"Kazama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunobu","family":"Hosokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideki","family":"Hishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01002749"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318999"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.28.1.33"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02428555"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12481882"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/8.4.290"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.127.3308.1240"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/6.6.425"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1974.tb01724.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/17.3.156"},{"key":"e_1_2_1_12_1","first-page":"233","article-title":"Morphological and morphochemical properties of the elastic system in the motor organ of man","volume":"3","author":"Gawlik Z.","year":"1965","journal-title":"Folia Histochem. Cytochem."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.2330\/joralbiosci1965.27.933"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01003406"},{"key":"e_1_2_1_15_1","first-page":"137A","article-title":"A formaldehyde\u2010glutaraldehyde fixative of high osmolality for use in electron microscopy (abstr)","volume":"27","author":"Karnovsky M. J.","year":"1965","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(58)90049-2"},{"key":"e_1_2_1_17_1","first-page":"23","article-title":"A histochemical comparison of human epithehal mucins in normal and in hypersecretory states including pancreatic cystic fibrosis","volume":"46","author":"Lev R.","year":"1965","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091390203"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/21.3.199"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/27.7.89157"},{"key":"e_1_2_1_21_1","first-page":"158","article-title":"A modified method for lead staining of thin sections","volume":"17","author":"Sato T.","year":"1968","journal-title":"J. Electron Microsc."},{"key":"e_1_2_1_22_1","first-page":"212","volume-title":"Dental Anatomy and Embryology. Book 2","author":"Shellis P.","year":"1981"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01890991"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051220302"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/13.3.211"},{"key":"e_1_2_1_26_1","first-page":"251","volume-title":"The Connective Tissue, International Academy of Pathology Monograph","author":"Spicer S. S.","year":"1967"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(69)90033-1"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01682751"},{"key":"e_1_2_1_29_1","first-page":"1","article-title":"Die Farbung der Epithelfasern","volume":"19","author":"Unna P. G.","year":"1894","journal-title":"Dermatologica"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1092250404","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1092250404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T16:38:09Z","timestamp":1697906289000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1092250404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,12]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,12]]}},"alternative-id":["10.1002\/ar.1092250404"],"URL":"https:\/\/doi.org\/10.1002\/ar.1092250404","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,12]]}}}