{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:55:10Z","timestamp":1770994510918,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":4988,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fibronexus is a close transmembrane association between fibronectin filaments and actin microfilaments. It has been found at the surfaces of fibroblasts in tissue culture, as well as within contracting granulation tissue. This specialized connection has been proposed to play an important role in the adhesive properties of fibroblasts. The purpose of this study is to determine whether the fibronexus is present in other contracting tissues besides granulation tissue, specifically in Dupuytren's diseased tissue. Dupuytren's disease is a pathologic condition in which the palmar aponeurosis becomes shortened leading to irreversible flexion of the digits. Shortening of the aponeurosis is believed to be an active cellular process. Extracellular filaments and actin microfilaments form close transmembrane associations at the surfaces of actin\u2010rich fibroblasts in Dupuytren's disease. Extracellular filaments extend from the cell surface into the surrounding tissue connecting fibroblasts with collagen fibrils and adjacent cells. In this study we have used immunoelectron microscopy to demonstrate that the extracellular filaments that participate in these close transmembrane associations contain fibronectin. High voltage electron microscopy has been used to examine the three\u2010dimensional relationships between the cytoskeleton and fibronectin filaments in Dupuytren's diseased tissue. We propose that the fibronexus is a dominant adhesive structure at the surface of fibroblasts in Dupuytren's diseased tissue. The fibronexus, by mediating cell\u2010to\u2010cell and cell\u2010to\u2010matrix attachments, may serve to transmit contractile forces generated by actin microfilaments in these cells throughout the diseased tissue.<\/jats:p>","DOI":"10.1002\/ar.1092300205","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T20:32:49Z","timestamp":1109449969000},"page":"175-182","source":"Crossref","is-referenced-by-count":48,"title":["Fibronectin filaments and actin microfilaments are organized into a fibronexus in Dupuytren's diseased tissue"],"prefix":"10.1002","volume":"230","author":[{"given":"James J.","family":"Tomasek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol J.","family":"Haaksma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.2.393"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90123-X"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.4.1103"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.5.1649"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0363-5023(78)80112-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.5.1738"},{"key":"e_1_2_1_8_1","first-page":"252","article-title":"Evidence for the non\u2010muscle nature of the \u201cmyofibroblast\u201d of granulation tissue and hypertrophic scar","volume":"130","author":"Eddy R. J.","year":"1988","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.12.7014712"},{"key":"e_1_2_1_10_1","first-page":"131","article-title":"Dupuytren's contracture: Fibroblast contraction? An ultrastructural study","volume":"66","author":"Gabbiani G.","year":"1972","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.2106\/00004623-198062030-00012","article-title":"Dupuytren's contracture. An electron microscopic, biochemical and clinical correlative study","volume":"62","author":"Gelberman R. H.","year":"1980","journal-title":"J. Bone Joint Surg."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12525694"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90300-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/320531a0"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90233-9"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90272-6"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1982.046.01.062"},{"key":"e_1_2_1_18_1","first-page":"137a","article-title":"A formaldehyde\u2010glutaraldehyde fixative of high osmolarity for use in electro microscopy","volume":"27","author":"Karnovsky M. J.","year":"1965","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90365-7"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.2106\/00004623-195941040-00008","article-title":"Dupuytren's contracture. A new concept of the pathogenesis correlated with surgical management","volume":"41","author":"Luck J. V.","year":"1959","journal-title":"J. Bone Joint Surg."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90040-0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/jcs.56.1.1","article-title":"Fibronexus formation is an early event during fibronectin\u2010induced restoration of more normal morphology and substrate adhesion patterns in transformed hamster fibroblasts","volume":"56","author":"Singer I. I.","year":"1982","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.6.2091"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90339-1"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.6.2171"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90744-0"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0363-5023(86)80143-5"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.2106\/00004623-198769090-00013","article-title":"Extracellular matrix\u2010cytoskeletal connections at the surface of the specialized contractile fibroblast (myofibroblast) in Dupuytren's disease","volume":"69","author":"Tomasek J. J.","year":"1987","journal-title":"J. Bone Joint Surg."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0174-173X(88)80027-X"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/275179a0"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.4.1217"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.1092300205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.1092300205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:44:09Z","timestamp":1698021849000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ar.1092300205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,6]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,6]]}},"alternative-id":["10.1002\/ar.1092300205"],"URL":"https:\/\/doi.org\/10.1002\/ar.1092300205","archive":["Portico"],"relation":{},"ISSN":["0003-276X","1097-0185"],"issn-type":[{"value":"0003-276X","type":"print"},{"value":"1097-0185","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,6]]}}}