{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:54:41Z","timestamp":1761216881084},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":4958,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The secretory granules of salivary glands often display complex internal substructures, yet little is known of the molecular organization of their contents or the mechanisms involved in packaging of the secretory proteins. We used post\u2010embedding immunogold labeling with antibodies to two secretory proteins, agglutinin and \u03b1\u2010amylase, to determine their distribution in the Golgi apparatus and secretory granules of the human submandibular gland acinar cells. With monoclonal antibodies specific for carbohydrate epitopes of the agglutinin, reactivity was found in the <jats:italic>trans Golgi<\/jats:italic> saccules, <jats:italic>trans Golgi<\/jats:italic> network, and immature and mature secretory granules. In the granules, labeling was seen in regions of low and medium electron density, but not in the dense cores. Reactivity seen on the apical and basolateral membranes of acinar and duct cells was attributed to a shared epitope on a membrane glycoprotein. Labeling with a polyclonal antibody to amylase was found in the Golgi saccules, immature and mature secretory granules, but not in the <jats:italic>trans Golgi<\/jats:italic> network. In the granules, amylase was present in the dense cores and in areas of medium density, but not in the regions of low density. These results indicate that these two proteins are distributed differently within the secretory granules, and suggest that they follow separate pathways between the Golgi apparatus and forming secretory granules. Small vesicles and tubular structures that labeled only with the antibodies to the agglutinin were observed on both faces of the Golgi apparatus and in the vicinity of the cell membrane. These structures may represent constitutive secretion vesicles involved in transport of the putative membrane glycoprotein to the cell membrane.<\/jats:p>","DOI":"10.1002\/ar.1092300303","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T20:35:47Z","timestamp":1109450147000},"page":"307-318","source":"Crossref","is-referenced-by-count":30,"title":["Differential distribution of salivary agglutinin and amylase in the Golgi apparatus and secretory granules of human salivary gland acinar cells"],"prefix":"10.1002","volume":"230","author":[{"given":"Kunio","family":"Takano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meredith","family":"Bogert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Malamud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Lally","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur R.","family":"Hand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90210-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/30.1.6172469"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01003438"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001850213"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.2.7354212"},{"key":"e_1_2_1_7_1","first-page":"102","volume-title":"Cell Biology of the Secretory Process","author":"Bennett G.","year":"1984"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/S0021-9258(19)75937-7","article-title":"Y and blood group B type 2 glycolipid antigens accumulate in a human gastric carcinoma cell line as detected by monoclonal antibody. 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