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Communication was observed and quantitated with microspectrofluorometric and image analysis techniques following scrape\u2010loading of the cells with lucifer yellow. Lucifer yellow migrated as far as ten cells away from the scraped edge in control populations. Two minute TPA (25\u201350 \u03bcg\/ml) treatment inhibited dye movement such that the dye remained mainly in the cells at the cut edge. The TPA\u2010induced inhibition of cell\u2010cell communication could be partially blocked by 15 min treatment of the cell populations with the PKC inhibitors trifluoperazine (30 \u03bcg\/ml), staurosporine (2 \u00d7 10<jats:sup>\u22128<\/jats:sup> or 2 \u00d7 10<jats:sup>\u22126<\/jats:sup> M), sangivamycin (15 or 200 \u03bcM), or a PKC inhibitor peptide (20 \u03bcg\/ml) scraped in at the same time as lucifer yellow. Normal communication was observed in cultures treated only with PKC inhibitors. Lower concentrations of TPA (50 ng\/ml\u20101 \u03bcg\/ml) used for 2 min did not inhibit dye communication. Our results demonstrate the phorbol ester\u2010induced interruption of cell\u2010cell communication. The inhibition of PKC by inhibitors eliminates the effect of TPA on communication. Our data are consistent with a role of PKC in the control of junctional communication. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.1092350102","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T20:01:15Z","timestamp":1109448075000},"page":"1-11","source":"Crossref","is-referenced-by-count":7,"title":["Regulation of the 12\u2010o\u2010tetradecanoyl\u2010phorbol\u201013\u2010acetate\u2010induced inhibition of intercellular communication"],"prefix":"10.1002","volume":"235","author":[{"given":"Sandra A.","family":"Murray","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Howard K.","family":"Plummer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"Edward E.","family":"Leonard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pushpa","family":"Deshmukh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34459-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4476"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/6.9.1353"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/325060a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90014-0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/283521a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.9.5628"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90195-X"},{"key":"e_1_2_1_10_1","first-page":"3614","article-title":"Rapid and reversible inhibition of junctional communication of tumor promoters in a mouse cell line","volume":"43","author":"Fitzgerald D. 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