{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T08:49:04Z","timestamp":1788425344734,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T00:00:00Z","timestamp":1106697600000},"content-version":"vor","delay-in-days":3831,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Background<\/jats:italic>: Fish cytotoxic effectors form a cell population whose ultrastructure and properties of conjugation with target cells have not been completely established. We report the ultrastructure of the non\u2010specific cytotoxic cells in a seawater teleost (<jats:italic>Sparus aurata<\/jats:italic> L.) and compare it to a freshwater species (<jats:italic>Cyprinus carpio<\/jats:italic> L.).<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: Blood leucocytes were incubated with HeLa or B16 melanoma cells. Samples were processed for transmission electron microscopic study.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: Conjugates consisting of leucocytes binding targets were regularly observed after 30 min, 1 hr, or 2 hr of incubation. In both species leucocytes binding to targets showed ultrastructural features of either monocyte\u2010like or lymphocyte\u2010like cells. Monocyte\u2010like cells usually appeared flattened against the targets and seemed to enclose fragments of the target to form cytoplasmic vesicles and the content of their scarce cytoplasmic granules seemed to be delivered into these vesicles. In the seabream lymphocyte\u2010like cells, dense cytoplasmic granules occurred only occasionally, and neither microvilli nor cell processes were present at the contact areas with the targets. In the carp, the contacts were more numerous and formed regularly interdigitating contact areas and the lymphocytes showed granules with characteristic dense and fibrillar contents.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>: We conclude that seabream and carp have a leucocyte cell population with ultrastructural features of either monocytes or lymphocytes showing nonspecific cytotoxic ability. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.1092390412","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T19:35:21Z","timestamp":1109446521000},"page":"468-474","source":"Crossref","is-referenced-by-count":29,"title":["Ultrastructure of nonspecific cytotoxic cells in teleosts. I. Effector\u2010target cell binding in a marine and a freshwater species (Seabream: <i>Sparus aurata<\/i> L., and Carp: <i>Cyprinus carpio<\/i> L.)"],"prefix":"10.1002","volume":"239","author":[{"given":"Jose","family":"Meseguer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Angeles","family":"Esteban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Lopez\u2010ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Bielek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,26]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1","article-title":"Influence of environmental temperature on the immune response in fish","volume":"61","author":"Avtalion R. R.","year":"1973","journal-title":"Curr. 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