{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:54:22Z","timestamp":1767858862590,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T00:00:00Z","timestamp":1180569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In many mammals, sperm associations had been observed, but not in the mouse. In this work, mouse sperm rosettes are morphologically described inside the epididymis and during its dissolution in a culture medium. Also characterized are the saccharides present in the linking material. Sperm association and other epididymal actions are supported by sperm during epididymal transit and are verified at the caudal region, suggesting a relation between epididymal transit and sperm maturation. In drops of epididymal content obtained from distal (cauda), but not from proximal (caput and corpus) regions; dissolved in culture medium, rosettes appear to be 10 to 15 motile sperm joined by their heads. After 3 min, sperm progressively detach, disassembling the rosette. These structures are studied by several techniques, including optic, electronic (scanning electron microscopy and transmission electron microscopy), and video microscopy. At the ultrastructural level, a dense network of electron\u2010dense material was observed between sperm heads, joining them. Based on previous works in rat, several lectins were used to characterize the type of saccharides present in this linking material. To avoid the contact between sperm and epididymal fluid from distal region\u2014that probably exerts an influence on sperm association\u2014a ligature was placed between caput and corpus. This epididymal content isolated from caput did not display any rosettes after 28 days. Anat Rec, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.20555","type":"journal-article","created":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T11:50:15Z","timestamp":1180612215000},"page":"814-824","source":"Crossref","is-referenced-by-count":11,"title":["Mouse Sperm Rosette: Assembling During Epididymal Transit, in vitro Disassemble, and Oligosaccharide Participation in the Linkage Material"],"prefix":"10.1002","volume":"290","author":[{"given":"Mar\u00eda De Los \u00c1ngeles","family":"Monclus","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre\u00edna","family":"Cesari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Eugenia","family":"Cabrillana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola Vanina","family":"Borelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Edith","family":"Vincenti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario H\u00e9ctor","family":"Burgos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel Walter","family":"Forn\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,31]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"7","volume-title":"The spermatozoon","author":"Bedford JM","year":"1979"},{"key":"e_1_2_6_3_1","first-page":"96","volume-title":"Colloidal gold: principles, methods and applications","author":"Benhamou N","year":"1989"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/208402b0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-3-839"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1995.tb01751.x"},{"key":"e_1_2_6_7_1","unstructured":"BurgosMH Forn\u00e9sMW Guti\u00e9rrezLS VincentiAE.1992. El proceso de maduraci\u00f3n del espermatozoide durante el tr\u00e1nsito epididimario. V Iberoamerican Congress of Cell Biology (Malaga) 16 (Reprod) 70."},{"key":"e_1_2_6_8_1","first-page":"254","article-title":"The androgen dependency of the process of sperm maturation in the Guinea pig","volume":"21","author":"Cameo MS","year":"1971","journal-title":"Acta Physiol Lat Am"},{"key":"e_1_2_6_9_1","first-page":"119","article-title":"Interactions between epididymal secretions and spermatozoa","volume":"53","author":"Cooper TG","year":"1998","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03285.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0670181"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00350481"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092290205"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1073"},{"key":"e_1_2_6_15_1","first-page":"115","article-title":"Sperm associations in the rat epididymis","volume":"14","author":"Forn\u00e9s MW","year":"1990","journal-title":"Microsc Electron Biol Celular"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080380108"},{"key":"e_1_2_6_17_1","first-page":"82","article-title":"Post\u2010testicular sperm environment and fertility [review]","author":"Gatti JL","year":"2004","journal-title":"Anim Reprod Sci"},{"key":"e_1_2_6_18_1","first-page":"298","volume-title":"Colloidal gold: principles, methods and applications","author":"Hacker GW","year":"1989"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-333927-0.50007-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.2.325"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0790153"},{"key":"e_1_2_6_22_1","first-page":"73","article-title":"Plasma membrane structure and remodeling during sperm maturation in the epididymis","volume":"53","author":"Jones R","year":"1998","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092080206"},{"key":"e_1_2_6_24_1","first-page":"89","volume-title":"Methods in cell biology","author":"Lutz DA","year":"1986"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0210167"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091750309"},{"key":"e_1_2_6_27_1","first-page":"33","article-title":"Membrane transformation in Golgi apparatus of rat spermatids. A role for thick cisternae and two classes of coated vesicles in acrosome formation","volume":"27","author":"Mollenhauer HH","year":"1976","journal-title":"J Microsc Electron Biol Celular"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.4.947"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00832"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2006.05.011"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120180104"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2003.tb02713.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1071\/ZO9570223"},{"key":"e_1_2_6_34_1","first-page":"163","article-title":"A study of the function of the epididymis. IV. The fate of non\u2010ejaculated spermatozoa in the genital tract of the male guinea pig","volume":"8","author":"Simeone FA","year":"1931","journal-title":"J Exp Biol"},{"issue":"1","key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1042\/bj2770213","article-title":"Asparagine\u2010linked glycoprotein biosynthesis in rat epididymis. Presence of a mannosidase II\u2010like enzyme","volume":"277","author":"Skudlarek MD","year":"1991","journal-title":"Biochem J"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.5057"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092360307"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.007856"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10313"},{"key":"e_1_2_6_40_1","first-page":"85","article-title":"Role of luminal fluid glycosyltransferases and glycosidases in the modification of rat sperm plasma membrane glycoproteins during epididymal maturation","volume":"53","author":"Tulsiani DR","year":"1998","journal-title":"J Reprod Fertil"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod23.4.877"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.4.1129"},{"issue":"3","key":"e_1_2_6_43_1","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1042\/bj2900743","article-title":"The structural basis of the inhibition of human alpha\u2010mannosidases by azafuranose analogues of mannose","volume":"290","author":"Winchester B","year":"1993","journal-title":"Biochem J"},{"key":"e_1_2_6_44_1","volume-title":"Mammalians fertilization","author":"Yanagimachi R","year":"1994"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0460049"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.20555","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.20555","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:20:22Z","timestamp":1759947622000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.20555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,31]]},"references-count":44,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1002\/ar.20555"],"URL":"https:\/\/doi.org\/10.1002\/ar.20555","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,31]]}}}