{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:41:12Z","timestamp":1791862872308,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T00:00:00Z","timestamp":1259712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is believed that estrogen deficiency is one of the major risk factors associated with osteoporosis. To investigate the effects of the transplantation of microencapsulated ovarian cells in estrogen\u2010deficient mice, ovarian cells from female Kunming (KM) mice (6\u2010weeks old) were separated, cultured, and microencapsulated with alginic acid\u2010polylysine\u2010alginic acid. Female KM mice (8\u2010weeks old) were randomly separated into three groups: intact (normal), ovariectomized (OVX), and treatment (OVX+ implantation). Microencapsulated ovarian cells were found to secrete estrogen at normal levels <jats:italic>in vitro<\/jats:italic>. Ninety days after transplantation, serum estradiol levels in the OVX group were significantly lower, and the trabecular bone amount and volume were decreased when compared with the normal group. The expression of alkaline phosphatase in chondrocytes appeared lower, while the expression of matrix metalloproteinase 9 (MMP\u20109) in the bone matrix was higher. The ratio of MMP\u20109\u2010positive chondrocytes and osteoblasts to osteoclasts was significantly lower than that of the normal group. The concentrations of hydroxyproline (Hyp), Ca, and P in the left femurs of the OVX group were lower than those of the normal group. However, the aforementioned changes were not seen in the treatment group. In conclusion, microencapsulated ovarian cells survive well after transplantation and secrete estrogen <jats:italic>in vivo<\/jats:italic>, and they can prevent in some degree osteoporosis caused by ovariectomy. Anat Rec, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.21036","type":"journal-article","created":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T10:10:25Z","timestamp":1259748625000},"page":"200-207","source":"Crossref","is-referenced-by-count":13,"title":["Prevention of Osteoporosis in Mice after Ovariectomy via Allograft of Microencapsulated Ovarian 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