{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:58:45Z","timestamp":1759971525924,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,7,22]],"date-time":"2010-07-22T00:00:00Z","timestamp":1279756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ciliary zonules are responsible for changing the curvature of a lens in the dioptric focus of an eye. Present established theory is based on the relaxation of zonular superficial fasciculi affixed to the capsular surface, thereby inducing the change of anterior\u2010 and posterior lens curvature causing spontaneous liquid movement of lens material. To achieve precise focusing at any distance, a more active functional organization should exist. The present studies were performed to determine not only the surface attachment but also the intracapsular affix of zonules on monkey eyes. In addition, the development of focusing in newborn and presbyopia is analyzed. Histology was prepared by conventional and molecular immunofluorescence stainings on the compositions of zonules with fibrillin\u20101 (FBN 1) and lens capsule with collagen IV (COL IV), and <jats:italic>in situ<\/jats:italic> hybridization (ISH) analyses on frozen sections. Superficial circumferential attachments of zonule were found radially oriented between ciliary processes and anterior\/posterior lens capsules forming a triangular figure. Two functional intralayer integrations were found above them; anterior\u2010posterior crossed fibers over the equator and radial fibers distributed toward the anterior or posterior polar areas. These fibers were bound tightly to the deep layer connective tissues close to the lens epithelium. Fine zonular fibers were aggregated, gradually forming bundles and bifurcated again on the way to the capsule. The lateral striped staining pattern in bundles suggested their elastic nature. Response of \u03b1\u2010helixes of collagen IV immunostaining was more positive on \u03b1\u20101,2,4 than \u03b1\u20103,5,6 on anterior\u2010 and posterior lens capsules. Newborn eyes revealed not fascicular but fine membranous zonules on the lens surface and small ciliary processes. ISH analysis revealed high synthetic expression of FBN 1 mRNA in cytoplasm of nonpigmented epithelial cells of ciliary processes. The synthetic expression of FBN 1 declined with aging. According to the mechanism of accommodation, active dynamic movement of anterior or posterior capsules play the main role of changing the lens configuration by two intralayer zonular integrations, including anterior\u2010posterior crossed fibers over the equator and radial fibers toward anterior or posterior polar areas acting with coordinated contraction of circular or longitudinal ciliary muscles. The developmental change on focusing is brought about by synthesis of FBN 1 in the newborn eye. Anat Rec 293:1797\u20131804, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.21220","type":"journal-article","created":{"date-parts":[[2010,7,22]],"date-time":"2010-07-22T10:00:20Z","timestamp":1279792820000},"page":"1797-1804","source":"Crossref","is-referenced-by-count":13,"title":["Intracapsular Organization of Ciliary Zonules in Monkey Eyes"],"prefix":"10.1002","volume":"293","author":[{"given":"Mari","family":"Hiraoka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2010Ichi","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiaki","family":"Ohtaka\u2010Maruyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunji","family":"Ohsako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naosuke","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruki","family":"Senoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Takada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9,16]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"553","article-title":"Immunohistologic studies of type IV collagen in anterior lens capsules of patients with Alport syndrome","volume":"70","author":"Cheong HI","year":"1994","journal-title":"Lab Invest"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1977.tb05678.x"},{"key":"e_1_2_6_4_1","series-title":"System of Ophthalmology","first-page":"180","author":"Duke\u2010Elder S","year":"1970"},{"key":"e_1_2_6_5_1","first-page":"365","article-title":"Fibrillin\u2010rich microfibrils: structural modifications during ageing in normal human zonule","volume":"30","author":"Hanssen E","year":"1998","journal-title":"J Submicrosc Cytol Pathol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(01)00122-6"},{"key":"e_1_2_6_7_1","first-page":"565","article-title":"Immunohistochemical study of the extracellular matrices related to accommodation in monkeys","volume":"106","author":"Hiraoka M","year":"2002","journal-title":"J Jap Ophthalmol Soc"},{"key":"e_1_2_6_8_1","first-page":"370","article-title":"Morphological study of the crystalline lens in monkeys","volume":"110","author":"Hiraoka M","year":"2006","journal-title":"J Jap Ophthalmol Soc"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-5155(98)00031-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(02)00014-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(92)90051-S"},{"key":"e_1_2_6_12_1","first-page":"84","article-title":"A comparative histologic study of the fibrillin microfibrillar system in the lens capsule of normal subjects and subjects with Marfan syndrome","volume":"39","author":"Mir S","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.121.6.846"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21350"},{"key":"e_1_2_6_15_1","first-page":"851","article-title":"The fine structure of the ciliary zonule and ciliary epithelium. With special regard to the organization and insertion of the zonular fibrils","volume":"10","author":"Raviola G","year":"1971","journal-title":"Invest Ophthalmol"},{"key":"e_1_2_6_16_1","first-page":"133","article-title":"Scanning electron microscopic studies of the zonular apparatus in human and monkey eyes","volume":"18","author":"Rohen JW","year":"1979","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_17_1","first-page":"1","volume-title":"Presbyopia: Cause and Treatment","author":"Schachar RA","year":"2001"},{"key":"e_1_2_6_18_1","first-page":"823","article-title":"The nature of the ocular zonule","volume":"130","author":"Streeten BW","year":"1982","journal-title":"Tr Am Ophth Soc"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.21220","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.21220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:40:48Z","timestamp":1759934448000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.21220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,16]]},"references-count":17,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1002\/ar.21220"],"URL":"https:\/\/doi.org\/10.1002\/ar.21220","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2010,9,16]]}}}