{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T20:25:43Z","timestamp":1770841543624,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T00:00:00Z","timestamp":1292976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The bioactive protein components from snake venom complexes have been utilized for studies of enzymology, structural biology, and pharmacology. The <jats:italic>Gloydius shedaoensis<\/jats:italic> snake (GSS) is the only snake species found exclusively at the Chinese Shedao (snake) Island in Dalian. To investigate the protein components of Chinese GSS venom (GSSV), we initialized a proteomic assay for GSSV by the combination of sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis with high\u2010performance liquid chromatography (HPLC)\u2010nanoelectrospray ionization tandem mass spectrometry (nESI\u2010MS\/MS). Thirty gel bands visualized by Coomassie blue staining were excised and digested by trypsin. The tryptic\u2010digested peptides were separated by HPLC and subsequently sequenced by nESI\u2010MS\/MS. Twenty\u2010four types of proteins were identified by searching the mass spectrometry data against NCBInr database through TurboSequest Bioworks. The most abundant proteins are phospholipase A<jats:sub>2<\/jats:sub>, metalloproteinase, <jats:sc>L<\/jats:sc>\u2010amino acid oxidase (LAAO), serine protease\/thrombin\u2010like enzyme. Except for 20 types of known snake venom proteins, the homolog peptides of hypothetical protein PFLC2230, LOC495267 protein, DEAD\/DEAH box helicase\u2010like, and pancreatic trypsin 1 from other organisms are matched for GSSV protein components. Mass spectrometric data also indicated that (<jats:italic>i<\/jats:italic>) dimerization happens to PLA<jats:sub>2<\/jats:sub>s as monomer and dimer of PLA<jats:sub>2<\/jats:sub>s coexist in GSSV and (<jats:italic>ii<\/jats:italic>) truncation or hydrolysis might happen to LAAOs as three molecular\u2010weight\u2010ranged LAAO species are present in GSSV. The results provide an \u201canatomical\u201d view of the protein composition and important information for protein characteristics of GSSV. Anat Rec, 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.21322","type":"journal-article","created":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T19:19:04Z","timestamp":1293045544000},"page":"273-282","source":"Crossref","is-referenced-by-count":15,"title":["\u201cAnatomical\u201d View of the Protein Composition and Protein Characteristics for <i>Gloydius shedaoensis<\/i> Snake Venom via Proteomics 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