{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:03:07Z","timestamp":1759971787073,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies indicated that G protein couple receptor 30 (GPR30), a nongenomic estrogen receptor, is widely expressed in many organ systems inducing many quick reaction of estrogen. However, there was rare report about the expression of GPR30 in the salivary gland. In the present study, we investigated the distribution of GPR30 in rat submaxillary gland by means of immunohistochemistry and <jats:italic>in situ<\/jats:italic> hybridization. GPR30 core sequences were amplified by RT\u2010PCR with total RNA extracted from rat submaxillary gland and were analyzed by sequencing with Sanger's method. The results showed that the epithelial cells of serous alveoli and granular convoluted duct in rat submaxillary gland displayed GPR30\u2010immunoreactivity on the plasma membrane and cytoplasm. Moreover, GPR30 mRNA hybridization signals were also detected in the cytoplasm of the above cells. GPR30 cDNA sequence cloned from rat submaxillary gland is identical to that of GPR30 from rat paraventricular and supraoptic nucleus. In conclusion, the expression of GPR30 in the serous and granular epithelial cells of submaxillary gland indicates that submaxillary gland could also be a target organ rapidly responding to estrogen stimulus, and estrogen may be involved in the functional regulation of submaxillary gland. Anat Rec, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.21349","type":"journal-article","created":{"date-parts":[[2011,3,3]],"date-time":"2011-03-03T04:09:19Z","timestamp":1299125359000},"page":"706-711","source":"Crossref","is-referenced-by-count":2,"title":["Histologic Distribution, Fragment Cloning, and Sequence Analysis of G Protein Couple Receptor 30 in Rat Submaxillary Gland"],"prefix":"10.1002","volume":"294","author":[{"given":"Lei","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Peng","family":"Mei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Jun","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gui\u2010He","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu\u2010De","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,3]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_6_2_1","DOI":"10.1677\/JOE-07-0017"},{"doi-asserted-by":"publisher","key":"e_1_2_6_3_1","DOI":"10.1677\/JOE-07-0392"},{"doi-asserted-by":"publisher","key":"e_1_2_6_4_1","DOI":"10.1006\/geno.1997.4972"},{"doi-asserted-by":"publisher","key":"e_1_2_6_5_1","DOI":"10.1210\/mend.14.10.0532"},{"doi-asserted-by":"publisher","key":"e_1_2_6_6_1","DOI":"10.1016\/j.bbrc.2006.05.191"},{"key":"e_1_2_6_7_1","first-page":"487","article-title":"Expression of estrogen receptor\u2010alpha protein in the rat digestive tract","volume":"21","author":"Gejima K","year":"2007","journal-title":"In Vivo"},{"doi-asserted-by":"publisher","key":"e_1_2_6_8_1","DOI":"10.1016\/j.neuroscience.2010.06.076"},{"doi-asserted-by":"publisher","key":"e_1_2_6_9_1","DOI":"10.1161\/HYPERTENSIONAHA.107.089995"},{"doi-asserted-by":"publisher","key":"e_1_2_6_10_1","DOI":"10.1677\/JOE-09-0066"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1097\/gme.0b013e3181d273c7","article-title":"Effect of dienogest on estrogen\u2010induced nitric oxide production in human umbilical vein endothelial cells and endothelium\u2010dependent vasodilatation in postmenopausal women","volume":"17","author":"Henmi N","year":"2010","journal-title":"Menopause"},{"doi-asserted-by":"publisher","key":"e_1_2_6_12_1","DOI":"10.1523\/JNEUROSCI.1158-10.2010"},{"doi-asserted-by":"publisher","key":"e_1_2_6_13_1","DOI":"10.1242\/dev.040659"},{"key":"e_1_2_6_14_1","first-page":"E522","article-title":"Dependence of tonin activity in rat submaxillary gland on growth hormone and testosterone","volume":"232","author":"Lis M","year":"1977","journal-title":"Am J Physiol"},{"doi-asserted-by":"publisher","key":"e_1_2_6_15_1","DOI":"10.1210\/en.2008-0623"},{"doi-asserted-by":"publisher","key":"e_1_2_6_16_1","DOI":"10.1210\/me.2008-0299"},{"doi-asserted-by":"publisher","key":"e_1_2_6_17_1","DOI":"10.1210\/en.2007-1663"},{"doi-asserted-by":"publisher","key":"e_1_2_6_18_1","DOI":"10.1016\/j.ygcen.2008.10.003"},{"key":"e_1_2_6_19_1","first-page":"125","article-title":"Effect of gonadectomy and adrenalectomy on steroid hormone content in rat submaxillary gland","volume":"18","author":"Paulo E","year":"1984","journal-title":"Endocrinol Exp"},{"doi-asserted-by":"publisher","key":"e_1_2_6_20_1","DOI":"10.1016\/j.jsbmb.2008.03.006"},{"doi-asserted-by":"publisher","key":"e_1_2_6_21_1","DOI":"10.1016\/j.steroids.2007.11.008"},{"doi-asserted-by":"publisher","key":"e_1_2_6_22_1","DOI":"10.1159\/000111559"},{"doi-asserted-by":"publisher","key":"e_1_2_6_23_1","DOI":"10.1126\/science.1106943"},{"doi-asserted-by":"publisher","key":"e_1_2_6_24_1","DOI":"10.1089\/dna.1990.9.553"},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1523\/JNEUROSCI.20-05-01694.2000","article-title":"Estrogen\u2010induced activation of the mitogen\u2010activated protein kinase cascade in the cerebral cortex of estrogen receptor\u2010alpha knock\u2010out mice","volume":"20","author":"Singh M","year":"2000","journal-title":"J Neurosci"},{"doi-asserted-by":"publisher","key":"e_1_2_6_26_1","DOI":"10.1016\/j.brainres.2009.11.004"},{"doi-asserted-by":"publisher","key":"e_1_2_6_27_1","DOI":"10.1210\/en.2004-1064"},{"doi-asserted-by":"publisher","key":"e_1_2_6_28_1","DOI":"10.1210\/me.2009-0120"},{"doi-asserted-by":"publisher","key":"e_1_2_6_29_1","DOI":"10.1016\/j.mce.2004.11.006"},{"doi-asserted-by":"publisher","key":"e_1_2_6_30_1","DOI":"10.1210\/me.2007-0359"},{"doi-asserted-by":"publisher","key":"e_1_2_6_31_1","DOI":"10.1210\/en.2008-0441"},{"doi-asserted-by":"publisher","key":"e_1_2_6_32_1","DOI":"10.1016\/j.neuroscience.2008.11.028"},{"doi-asserted-by":"publisher","key":"e_1_2_6_33_1","DOI":"10.1371\/journal.pone.0011791"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.21349","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.21349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:11:26Z","timestamp":1759943486000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.21349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1002\/ar.21349"],"URL":"https:\/\/doi.org\/10.1002\/ar.21349","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2011,3]]}}}