{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:30:56Z","timestamp":1786977056972,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,8]],"date-time":"2012-10-08T00:00:00Z","timestamp":1349654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Oxidative stress has been implicated as a cause of hair cell damage after ischemia reperfusion injury, noise trauma, and ototoxic injury. Oxidative stress can induce both apoptosis or necrosis depending on the degree of exposure. To study how reactive oxygen species (ROS) interacts with hair cells, we have developed an\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of oxidative stress using organ of Corti cultures exposed to physiologically relevant concentrations of hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ). Treatment of organ of Corti cultures with low concentrations of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    results in loss of outer hair cells in the basal turn of the explant. Higher concentrations of peroxide result in more extensive outer hair cell injury as well as loss of inner hair cells. Early outer hair cell death appears to occur though apoptosis as demonstrated by staining of activated caspase. The effect of oxidative stress on mitochondrial function is a key determinant of degree of damage. Oxidative stress results in reduction of the mitochondrial membrane potential and reduction of mitochondrial produced antioxidants. Low doses of oxidative stress induce changes in mitochondrial gene expression and induce mitochondrial DNA deletions. Recurrent oxidative stress or inhibition of mitochondrial function significantly enhanced hair cell death. This tissue culture model of oxidative hair cell injury maintains a pattern of injury similar to what is observed\n                    <jats:italic>in vivo<\/jats:italic>\n                    after oxidative injury and can be used to study the effects of ROS on hair cells over the time period of the culture. Anat Rec, 2012. \u00a9 2012 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/ar.22594","type":"journal-article","created":{"date-parts":[[2012,10,9]],"date-time":"2012-10-09T00:08:53Z","timestamp":1349741333000},"page":"1868-1876","source":"Crossref","is-referenced-by-count":44,"title":["Low Dose Oxidative Stress Induces Mitochondrial Damage in Hair Cells"],"prefix":"10.1002","volume":"295","author":[{"given":"Kim","family":"Baker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hinrich","family":"Staecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.1999.0229"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(96)00075-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(96)00126-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(00)00036-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199904000-00001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/0270-9139(95)90071-3","article-title":"In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note","volume":"21","author":"Grasl\u2010Kraupp B","year":"1995","journal-title":"Hepatology"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022403224901"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/000164800760370774"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(02)00286-1"},{"key":"e_1_2_6_11_1","unstructured":"HuB YangWP NicoteraT HendersonD.2003.Mitochondrial functions in apoptotic and necrotic OHCs following exposure to impulse noise. ARO MidWinter Meeting."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.22.3.165.5517"},{"key":"e_1_2_6_13_1","first-page":"559","article-title":"Use of organotypic cultures of Corti's organ to study the protective effects of antioxidant molecules on cisplatin\u2010induced damage of auditory hair cells","volume":"18","author":"Kopke RD","year":"1997","journal-title":"Am J Otol"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02733-5"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)04205-1"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000013845"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000013846"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(01)00224-6"},{"key":"e_1_2_6_19_1","unstructured":"YamashitaD JiangHY MillerJM SchachtJ.2003.Late mergence of free radicals after noise exposure. ARO MidWinter Meeting."},{"key":"e_1_2_6_20_1","unstructured":"YangWP BohneBA NicoteraT HendersonD.2003.Quantitative analysis of apoptotic and necrotic outer hair cells after exposure to different levels of continuous noise. ARO MidWinter Meeting."}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.22594","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.22594","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:30:04Z","timestamp":1759941004000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.22594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,8]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1002\/ar.22594"],"URL":"https:\/\/doi.org\/10.1002\/ar.22594","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,8]]}}}