{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:22:18Z","timestamp":1785522138202,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,5,27]],"date-time":"2013-05-27T00:00:00Z","timestamp":1369612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The process of water movement in the excurrent duct system of the male reproductive tract is pivotal for establishment of male fertility. The objective was to elucidate expression of aquaporin (AQP) water channels in the stallion reproductive tract. Real\u2010time RT\u2010PCR detected expression of <jats:italic>AQP0<\/jats:italic>\u2013<jats:italic>5<\/jats:italic> and AQP7\u201311 in testis, epididymis, and ductus deferens of mature stallions. There were two main expression patterns: (1) higher expression in testis than in epididymis and ductus deferens (<jats:italic>AQP<\/jats:italic>0, \u2212<jats:italic>4<\/jats:italic>, \u2212<jats:italic>5<\/jats:italic>, \u2212<jats:italic>8<\/jats:italic>, \u2212<jats:italic>10<\/jats:italic>, and \u2212<jats:italic>11<\/jats:italic>); and (2) lower expression in testis than in epididymis and ductus deferens (<jats:italic>AQP1<\/jats:italic>, \u2212<jats:italic>3<\/jats:italic>, \u2212<jats:italic>7<\/jats:italic>, and \u2212<jats:italic>9<\/jats:italic>). Overall, we inferred that fluid transport in the stallion testicle involved a collaboration of AQP subtypes (primarily AQP2, \u22125, \u22127, and \u22128). Based on immunohistochemistry, expression of AQP subtypes analyzed (i.e., AQP0, \u22122, \u22125, and \u22129) was localized to Leydig cells and elongated and round spermatids. Functional significance of AQP expression by Leydig cells remained uncertain. In elongated and round spermatids, AQP s likely contributed to the volume reduction observed during spermatogenesis. Subtypes AQP2 and AQP9 were the predominant forms expressed in epididymal tissue. Regulation of AQP2 expression, especially in the epididymal head, seemed to occur at the post\u2010transcriptional level, as protein expression upon immunohistochemistry was pronounced, despite low transcript abundance. In epididymal tissue, AQPs likely contributed to fluid resorbtion, given their localization on the apical membrane of principal cells. Anat Rec, 296:1115\u20131126, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.22709","type":"journal-article","created":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T05:23:48Z","timestamp":1402291428000},"page":"1115-1126","source":"Crossref","is-referenced-by-count":34,"title":["Region\u2010Specific Expression of Aquaporin Subtypes in Equine Testis, Epididymis, and Ductus Deferens"],"prefix":"10.1002","volume":"296","author":[{"given":"C.","family":"Klein","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine University of Calgary Calgary Alberta T2N 4Z6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.H.T.","family":"Troedsson","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, MH Gluck Equine Research Center 108 Gluck Equine Research Center Lexington Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Rutllant","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Western University of Health Sciences 309 East Second Street Pomona California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,27]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.020818"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02243.x"},{"key":"e_1_2_5_4_1","first-page":"264","article-title":"Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract","volume":"61","author":"Brown D","year":"1993","journal-title":"Eur J Cell 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