{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:34:16Z","timestamp":1760985256456,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T00:00:00Z","timestamp":1405468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Danish Society for ORL HNS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Common middle ear diseases may affect bone behavior in the middle ear air cell system. To understand this pathologic pneumatization, the normal development of bone in the middle ear should be investigated. The objective of this study was to analyze gene expression of bone\u2010related signaling factors and gene sets in the developing middle ear. Microarray technology was used to identify bone\u2010related genes and gene sets, which were differentially expressed between the lining tissue of adult (quiescent) bulla and young (resorbing\/forming) bulla. Data were analyzed using tools of bioinformatics and expression levels of selected genes were validated using quantitative polymerase chain reaction. The candidate gene products were compared with previously published data on middle ear bone metabolism. No differentially expressed genes were found on the outer surface of bulla. On the inner lining a total of 260 genes were identified of which 22 genes were involved in bone metabolism. Gene set analysis revealed five enriched bone\u2010related gene sets. The identified differentially expressed bone\u2010related mRNAs and gene sets are of potential significance in the normally developing bulla. These factors and gene sets may also play important roles during pathologic pneumatization of the middle ear air cell system in common middle ear diseases. In addition, this study suggests that the control of growth rate and wall thickness from resorptive as well as formative signals all originate from the inner lining cells of the bulla wall. Anat Rec, 297:2349\u20132355, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.22992","type":"journal-article","created":{"date-parts":[[2015,1,29]],"date-time":"2015-01-29T12:18:44Z","timestamp":1422533924000},"page":"2349-2355","source":"Crossref","is-referenced-by-count":5,"title":["Bone Signaling in Middle Ear Development: A Genome\u2010Wide Differential Expression Analysis"],"prefix":"10.1002","volume":"297","author":[{"given":"Michelle Christine","family":"Nielsen","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head &amp; Neck Surgery and Audiology, Rigshospitalet University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas Martin","family":"Bertelsen","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotech Research and Innovation Centre, The Bioinformatics Centre, Faculty of Science University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"Friis","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head &amp; Neck Surgery and Audiology, Rigshospitalet University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ole","family":"Winther","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotech Research and Innovation Centre, The Bioinformatics Centre, Faculty of Science University of Copenhagen Copenhagen Denmark"},{"name":"DTU Informatics Technical University of Denmark Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lennart","family":"Friis\u2010Hansen","sequence":"additional","affiliation":[{"name":"Center for Genomic Medicine, Rigshospitalet University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Cay\u00e9\u2010Thomasen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head &amp; Neck Surgery and Audiology, Rigshospitalet University of Copenhagen Copenhagen Denmark"},{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Gentofte Hospital University of Copenhagen Hellerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mads S\u00f8lvsten","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Gentofte Hospital University of Copenhagen Hellerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S002221510013419X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(98)00064-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm537"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-140"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199905000-00009"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni179"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1242\/dev.127.7.1337","article-title":"A model for anteroposterior patterning of the vertebrate limb based on sequential long\u2010 and short\u2010range Shh signalling and Bmp signalling","volume":"127","author":"Drossopoulou G","year":"2000","journal-title":"Development"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90476-J"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016489.2011.616910"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-9-117"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.241"},{"key":"e_1_2_6_15_1","first-page":"202","article-title":"Effect of tubal function on the osseous structures of the petrous bone","volume":"34","author":"Hormann K","year":"1986","journal-title":"An animal experiment study. 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