{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:48:15Z","timestamp":1759970895177,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE019629"],"award-info":[{"award-number":["DE019629"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>ClC\u20107 is a 2Cl<jats:sup>\u2212<\/jats:sup>\/1H<jats:sup>+<\/jats:sup>\u2010exchanger expressed at late endosomes and lysosomes, as well as the ruffled border of osteoclasts. ClC\u20107 deficiencies in mice and humans lead to impaired osteoclast function and therefore osteopetrosis. Failure of tooth eruption is also apparent in ClC\u20107 mutant animals, and this has been attributed to the osteoclast dysfunction and the subsequent defect in alveolar bone resorptive activity surrounding tooth roots. Ameloblasts also express ClC\u20107, and this study aims to determine the significance of ClC\u20107 in enamel formation by examining the dentitions of ClC\u20107 mutant mice. Micro\u2010CT analysis revealed that the molar teeth of 3\u2010week old ClC\u20107 mutant mice had no roots, and the incisors were smaller than their age\u2010matched controls. Despite these notable developmental differences, the enamel and dentin densities of the mutant mice were comparable to those of the wild\u2010type littermates. Scanning electron microscopy showed normal enamel crystallite and prismatic organization in the ClC\u20107 mutant mice, although the enamel was thinner (hypoplastic) than in controls. These results suggested that ClC\u20107 was not critical to enamel and dentin formation, and the observed tooth defects may be related more to a resulting alveolar bone phenotype. Micro\u2010CT analysis also revealed abnormal features in the calvarial bones of the mutant mice. The cranial sutures in ClC\u20107 mutant mice remained open compared to the closed sutures seen in the control mice at 3 weeks. These data demonstrate that ClC\u20107 deficiency impacts the development of the dentition and calvaria, but does not significantly disrupt amelogenesis. Anat Rec, 298:1502\u20131508, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23118","type":"journal-article","created":{"date-parts":[[2015,2,7]],"date-time":"2015-02-07T03:05:15Z","timestamp":1423278315000},"page":"1502-1508","source":"Crossref","is-referenced-by-count":9,"title":["Dental and Cranial Pathologies in Mice Lacking the <scp>C<\/scp>l<sup>\u2212<\/sup>\/<scp>H<\/scp><sup>+<\/sup>\u2010Exchanger <scp>C<\/scp>l<scp>C<\/scp>\u20107"],"prefix":"10.1002","volume":"298","author":[{"given":"Xin","family":"Wen","sequence":"first","affiliation":[{"name":"Herman Ostrow School of Dentistry of USC Center for Craniofacial Molecular Biology, University of Southern California Los Angeles USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo S.","family":"Lacruz","sequence":"additional","affiliation":[{"name":"Department of Basic Science and Craniofacial Biology College of Dentistry New York University New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L.","family":"Paine","sequence":"additional","affiliation":[{"name":"Herman Ostrow School of Dentistry of USC Center for Craniofacial Molecular Biology, University of Southern California Los Angeles 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