{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:53:14Z","timestamp":1759971194130,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,7,30]],"date-time":"2015-07-30T00:00:00Z","timestamp":1438214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"LOCTI","award":["2011000906"],"award-info":[{"award-number":["2011000906"]}]},{"name":"Mppeuct (Ministerio del Poder Popular para la Educacion Universitaria, Ciencia y Tecnologia)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Congenital pseudoarthrosis of the tibia (CPT) is an uncommon disease whose etiology and pathogenesis is unknown. Several evidences suggest that decreased osteogenic capacities, impaired local vascularization, and microenvironment alterations may play a role in the pathogenesis of CPT. Additionally, it is not clear if the pathogenesis of this disease is related to the absence of cells with osteogenic capacity of differentiation. In this work, a two\u2010year\u2010old patient diagnosed with CPT underwent an orthopedic surgery to promote bone union in a pseudoarthrosis lesion. Tissue from CPT lesion was excised, and histological evaluation and tissue culture were performed. Histologic analysis of the soft CPT lesion showed the presence of highly cellular fibrous tissue, vascularization, and abundant extracellular matrix. Fusiform cells of mesenchymal appearance were observed but osteoblasts, osteoclasts, chondrocytes, and adipose cells were not found. There was no evidence of osteogenesis. CPT tissue cultured as explants showed, after one month of culture, evidence of osteogenesis, chondrogenesis, and adipogenesis. Cells isolated from explants of CPT tissue showed a fibroblast\u2010like morphology and expressed the mesenchymal stromal cell (MSC) markers: CD105, CD73, and CD90 (CPT\u2010MSC). Functional analysis showed that CPT\u2010MSC differentiate, <jats:italic>in vitro<\/jats:italic>, into osteogenic, chondrogenic, and adipocytic cells. CPT\u2010MSC expressed osteocalcin and agrecan. CPT\u2010MSC produced collagen in the presence of ascorbic acid. MSC from BM of normal individuals were used as control. In summary, our results indicate that CPT tissue contains MSC with osteogenic capacity of differentiation. It is possible that CPT microenvironment may contribute to impair the osteogenic capacity of differentiation of CPT\u2010MSC. Anat Rec, 298:1804\u20131814, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23198","type":"journal-article","created":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T19:48:54Z","timestamp":1437421734000},"page":"1804-1814","source":"Crossref","is-referenced-by-count":7,"title":["Isolation and Characterization of Multipotential Mesenchymal Stromal Cells from Congenital Pseudoarthrosis of the Tibia: Case Report"],"prefix":"10.1002","volume":"298","author":[{"given":"Dylana","family":"Diaz\u2010Solano","sequence":"first","affiliation":[{"name":"Unidad de Terapia Celular\u2013Laboratorio de Patolog\u00eda Celular y Molecular, Centro de Medicina Experimental Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC) Apartado 21827 Caracas 1020\u2010A Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Wittig","sequence":"additional","affiliation":[{"name":"Unidad de Terapia Celular\u2013Laboratorio de Patolog\u00eda 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