{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T14:16:06Z","timestamp":1761401766942,"version":"build-2065373602"},"reference-count":70,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T00:00:00Z","timestamp":1444780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The cremaster muscle (CM) is a striated muscle showing some unusual features for ordinary striated muscles, in fact it receives, besides somatic innervation, a conspicuous autonomic sympathetic innervation. The autonomic neurons associated with the CM of 4 male intact pigs were typified combining the retrograde nontrans\u2010synaptic fluorescent tracer Fast Blue (FB) and double labeling immunohistochemical methods. We collected the L4 sympathetic trunk ganglion (STG), that our preliminary studies proved to contain the highest number (575.5\u2009\u00b1\u2009152.93; mean\u2009\u00b1\u2009S.E.M., <jats:italic>n =<\/jats:italic> 4) of FB+ sympathetic neurons projecting to CM. About half of the CM projecting neurons of this ganglion were catecholaminergic and showed the colocalization of Tyrosine Hydroxylase (TH) with Neuropeptide Y (NPY), Leu\u2010Enkephaline (LENK), Vasoactive Intestinal Polypeptide (VIP), Calcitonine Gene Related Peptide (CGRP), Substance P (SP), neuronal Nitric Oxyde Sinthase (n\u2010NOS), and Vesicular Acetylcholine Transporter (VAChT). The noncatecholaminergic neurons were immunoreactive for all the other markers tested, even if in small percentages. The conspicuous and heterogeneous contribution of the sympathetic autonomic neurons to the muscle innervation is consistent with the hypothesis of a possible origin of the CM fibers by transdifferentiation of the smooth muscle\u2010like gubernaculum mesenchyma into striated myotubes, suggesting that the cremaster myogenesis is independent from that of the abdominal muscles. Anat Rec, 298:2091\u20132097, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23271","type":"journal-article","created":{"date-parts":[[2015,9,24]],"date-time":"2015-09-24T12:03:04Z","timestamp":1443096184000},"page":"2091-2097","source":"Crossref","is-referenced-by-count":4,"title":["Neurochemical Features of the Autonomic Neurons Projecting to the Cremaster Muscle of the Boar"],"prefix":"10.1002","volume":"298","author":[{"given":"Maddalena","family":"Botti","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences University of Parma Parma 43126 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"Ragionieri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Parma Parma 43126 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Cacchioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Parma Parma 43126 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferdinando","family":"Gazza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Parma Parma 43126 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rino","family":"Panu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Parma Parma 43126 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(88)90038-6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijir.3900513"},{"volume-title":"Anatomia comparata dei Mammiferi domestici","year":"1983","author":"Barone R.","key":"e_1_2_7_4_1"},{"key":"e_1_2_7_5_1","first-page":"H 2693","article-title":"Direct coupling between blood flow and metabolism at the capillary level in striated muscle","volume":"272","author":"Berg BR","year":"1997","journal-title":"Am J Physiol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)64401-X"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20378"},{"key":"e_1_2_7_8_1","first-page":"65","article-title":"Peripheral neurons innervating the extrinsic smooth penile musculature of the pig: experimental study by retrograde transport and immunohistochemistry","volume":"111","author":"Botti M","year":"2006","journal-title":"It J Anat Embryol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20962"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22519"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2013.12.002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0530377"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655901750174791"},{"key":"e_1_2_7_14_1","first-page":"141","article-title":"Peptidergic innervation of the porcine oviduct studied by double\u2010labelling immunohistochemistry","volume":"34","author":"Czaja K","year":"1996","journal-title":"Folia Histochem Cytobiol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1243627"},{"key":"e_1_2_7_16_1","first-page":"247","article-title":"The pig model for biomedical research","volume":"41","author":"Dodds WJ.","year":"1982","journal-title":"Fed Proc"},{"volume-title":"Textbook of veterinary anatomy","year":"2002","author":"Dyce KM","key":"e_1_2_7_17_1"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(84)90064-8"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)60028-4"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20247"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2752(1996)17:7<402::AID-MICR10>3.0.CO;2-3"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:VERC.0000014227.55951.b9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(89)90116-1"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.14.1.197"},{"key":"e_1_2_7_25_1","first-page":"1087","volume-title":"Pediatric surgery","author":"Hutson JM.","year":"1998"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1984.sp015236"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19980915)42:6<409::AID-JEMT4>3.0.CO;2-H"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-002-0610-3"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(99)00084-4"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(99)00087-9"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1987.tb04574.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19960901)35:1<44::AID-JEMT5>3.0.CO;2-S"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000013783.63773.8F"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/294080a0"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1556\/AVet.58.2010.3.11"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-011-2983-4"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb48930.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10221"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-1281(11)80185-6"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0692-y"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11055-007-0020-9"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12025"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-10-73"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(85)90280-0"},{"key":"e_1_2_7_45_1","first-page":"97","article-title":"Synthesis and preliminary evaluation of a new 99mTc labeled substance P analogue as a potential tumor imaging agent","volume":"14","author":"Mozaffari S","year":"2015","journal-title":"Iranian J Pharmaceutical Res"},{"key":"e_1_2_7_46_1","first-page":"8","article-title":"Significance of Katp channels, L\u2010type Ca2+ channels and CYP450\u20104A enzymes in oxygen sensing in mouse cremaster muscle arterioles in vivo","volume":"13","author":"Ngo AT","year":"2013","journal-title":"Physiology"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/295202a0"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2007.05.017"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5243.1818"},{"key":"e_1_2_7_50_1","first-page":"1","article-title":"Co\u2010release and functional interactions of neuropeptide Y and noradrenaline in peripheral sympathetic vascular control","volume":"568","author":"Pernow J.","year":"1988","journal-title":"Acta Physiol Scand Suppl"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)90471-2"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.4081\/1183"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2013.e16"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23177"},{"key":"e_1_2_7_55_1","first-page":"173","article-title":"Some observations on the innervation of the striated muscle in the mouse esophagus \u2010 An electron microscope study","volume":"112","author":"Samarasinghe DY.","year":"1972","journal-title":"J Anat"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.04.012"},{"key":"e_1_2_7_57_1","first-page":"301","article-title":"Immunohistochemical properties of sympathetic chain ganglia (SChG) neurons projecting to the porcine testis in animals subjected to hemicastration, castration and testosterone supplementation","volume":"13","author":"Sienkiewicz W.","year":"2010","journal-title":"Pol J Vet Sci"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1556\/AVet.58.2010.2.5"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.64.1.112"},{"volume-title":"Swine as models in biomedical research","year":"1992","author":"Swindle MM","key":"e_1_2_7_60_1"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(00)70059-5"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2001.22285"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2001.23959"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2001.27042"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1159\/000065558"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(03)00574-8"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-837604"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00076.2004"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-1-00267.x"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.038984"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2002.00112.x"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.23271","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.23271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:22:46Z","timestamp":1759940566000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.23271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,14]]},"references-count":70,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1002\/ar.23271"],"URL":"https:\/\/doi.org\/10.1002\/ar.23271","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2015,10,14]]}}}