{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:08:02Z","timestamp":1759972082039,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T00:00:00Z","timestamp":1467763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan","award":["20592148","21592342","15K11022"],"award-info":[{"award-number":["20592148","21592342","15K11022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Xerostomia, or dry mouth, is a common syndrome that is generally treated with artificial saliva; however, no other effective methods have yet been established. Saliva secretion is mainly under the control of the autonomic nervous system. Pituitary adenylate cyclase\u2010activating polypeptide (PACAP) is recognized as a multifunctional neuropeptide in various organs. In this study, we examined the effect of PACAP on saliva secretion, and detected the distribution of the PACAP type 1 receptor (PAC1R) in major salivary glands, including the parotid, submandibular, and sublingual glands, in 9\u2010week\u2010old male C57BL\/6 mice. Intranasal administration of PACAP 38 increased the amount of saliva secreted, which was not inhibited by atropine pretreatment. Immunohistochemical analysis showed that PAC1R was distributed in the three major salivary glands. In the parotid and sublingual glands, PAC1R was detected in striated duct cells, whereas in the submandibular gland, a strong PAC1R immunoreaction was detected in tall columnar epithelial cells in the granular ducts (i.e., pillar cells), as well as in some striated duct cells. PACAP significantly increased the concentration of epidermal growth factor in saliva. These results suggest that PACAP directly regulates saliva secretion by controlling the absorption activity in the ducts, and that pillar cells regulate the function of granular epithelial cells in the granular duct, such as the secretion of growth factors into the saliva. Collectively, these results suggest the possibility of PACAP as a new effective treatment of xerostomia. Anat Rec, 299:1293\u20131299, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23388","type":"journal-article","created":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T04:17:15Z","timestamp":1466741835000},"page":"1293-1299","source":"Crossref","is-referenced-by-count":7,"title":["Pituitary adenylate cyclase\u2010activating polypeptide enhances saliva secretion via direct binding to PACAP receptors of major salivary glands in mice"],"prefix":"10.1002","volume":"299","author":[{"given":"Yuko","family":"Matoba","sequence":"first","affiliation":[{"name":"Department of Oral Anatomy and Developmental Biology Showa University School of Dentistry Shinagawa\u2010Ku Tokyo Japan"},{"name":"Division of Oral Surgery Yokohama General Hospital Aoba\u2010Ku Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Nonaka","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy and Developmental Biology Showa 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