{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:38:53Z","timestamp":1773949133669,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,9,10]],"date-time":"2016-09-10T00:00:00Z","timestamp":1473465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31272521"],"award-info":[{"award-number":["31272521"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions, China"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Spermatozoa are known to be stored in the epididymis of the Chinese soft\u2010shelled turtle <jats:italic>Pelodiscus sinensis<\/jats:italic> for long periods during hibernation, but the mechanism that underlies the sperm storage is poorly understood. This study was carried out to confirm the presence of TLR2\/4 (Toll\u2010like receptor 2\/4) in epididymal spermatozoa during the hibernation season and to analyze whether TLRs play a role in sperm storage. The structure and ultrastructure of a spermatozoon during the hibernation stage were investigated using light\u2010 and transmission electron\u2010microscopy. RT\u2010PCR was used to analyze mRNA expression, while protein expression was determined via Western blot. TLR2\/4 mRNA and proteins were detected in spermatozoa. Immunofluorescence staining was used to confirm TLR2\/4 localization in the spermatozoon, and TLR2\/4 were localized in the midpiece and the posterior segment of the head of the spermatozoon, which corresponded to the cytoplasmic droplets (CDs) of the turtle spermatozoon. As TLRs play critical roles in detecting and responding to invading pathogens, this study provided molecular evidence that TLR2\/4 might contribute to sperm storage in the epididymides. Anat Rec, 299:1578\u20131584, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23463","type":"journal-article","created":{"date-parts":[[2016,8,17]],"date-time":"2016-08-17T11:32:42Z","timestamp":1471433562000},"page":"1578-1584","source":"Crossref","is-referenced-by-count":15,"title":["Expression of TLR2\/4 on Epididymal Spermatozoa of the Chinese Soft\u2010Shelled Turtle <i>Pelodiscus sinensis<\/i> During the Hibernation Season"],"prefix":"10.1002","volume":"299","author":[{"given":"Lisi","family":"Hu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanfu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jameel A.","family":"Gandahi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology Sindh Agriculture University Tandojam Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamseel S.","family":"Arain","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology Sindh Agriculture University Tandojam Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Le","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tengfei","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University Nanjing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Y. 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