{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:05:45Z","timestamp":1759971945128,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,11,25]],"date-time":"2016-11-25T00:00:00Z","timestamp":1480032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Parent\/Bridge programme of the Foundation for Polish Science, co-financed by the European Union under the European Regional Development Fund"},{"name":"National Science Center","award":["2012\/07\/B\/NZ7\/04183"],"award-info":[{"award-number":["2012\/07\/B\/NZ7\/04183"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cell therapy is emerging as an alternative treatment of stress urinary incontinence. However, many aspects of the procedure require further optimization. A large animal model is needed to reliably test cell delivery methods. In this study, we aim to determine suitability of the goat as an experimental animal for testing intraurethral autologous cell transplantation in terms of urethral anatomy and cell culture parameters. The experiments were performed in 12 mature\/aged female goats. Isolated caprine muscle derived cells (MDC) were myogenic <jats:italic>in vitro<\/jats:italic> and mesenchymal stem cells (MSC) population was able to differentiate into adipo\u2010, osteo\u2010 and chondrogenic lineages. The median yield of cells after 3 weeks of culture amounted 47 \u00d7 10(6) for MDC and 37 \u00d7 10(6) for MSC. Urethral pressure profile measurements revealed the mean functional urethral length of 3.75\u2009\u00b1\u20090.7 cm. The mean maximal urethral closure pressure amounted 63.5\u2009\u00b1\u20095.9 cmH<jats:sub>2<\/jats:sub>O and the mean functional area was 123.3\u2009\u00b1\u200919.4 cm*cmH<jats:sub>2<\/jats:sub>O. The omega\u2010 shaped striated urethral sphincter was well developed in the middle and distal third of the urethra and its mean thickness on cross section was 2.3 mm. In the proximal part of the urethra only loosely arranged smooth muscle fibers were identified. To conclude, presented data demonstrate that caprine MDC and MSC can be expanded <jats:italic>in vitro<\/jats:italic> in a repeatable manner even when mature or aged animals are cell donors. Results suggest that female caprine urethra has similar parameters to those reported in human and therefore the goat can be an appropriate experimental animal for testing intraurethral cell transplantation. Anat Rec, 00:000\u2013000, 2016. \u00a9 2016 Wiley Periodicals, Inc. Anat Rec, 300:577\u2013588, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23498","type":"journal-article","created":{"date-parts":[[2016,10,14]],"date-time":"2016-10-14T18:57:15Z","timestamp":1476471435000},"page":"577-588","source":"Crossref","is-referenced-by-count":12,"title":["The Anatomy of Caprine Female Urethra and Characteristics of Muscle and Bone Marrow Derived Caprine Cells for Autologous Cell Therapy Testing"],"prefix":"10.1002","volume":"300","author":[{"given":"Anna","family":"Burdzinska","sequence":"first","affiliation":[{"name":"Department of Immunology Transplantology and Internal Medicine, Transplantation Institute, Medical University of Warsaw Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bartosz","family":"Dybowski","sequence":"additional","affiliation":[{"name":"Department of Urology Medical University of Warsaw Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weronika","family":"Zarychta\u2010Wisniewska","sequence":"additional","affiliation":[{"name":"Department of Immunology Transplantology and Internal Medicine, Transplantation Institute, Medical University of Warsaw Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Kulesza","sequence":"additional","affiliation":[{"name":"Department of Immunology Transplantology and Internal Medicine, Transplantation Institute, Medical University of Warsaw Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radoslaw","family":"Zagozdzon","sequence":"additional","affiliation":[{"name":"Department of Immunology Center of Biostructure Research, Medical University of Warsaw Warsaw Poland"},{"name":"Department of Bioinformatics Institute of Biochemistry and Biophysics, Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zdzislaw","family":"Gajewski","sequence":"additional","affiliation":[{"name":"Department of Large Animal Diseases with Clinic Faculty of Veterinary Medicine Warsaw University of Life Sciences (SGGW) Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leszek","family":"Paczek","sequence":"additional","affiliation":[{"name":"Department of Immunology Transplantology and Internal Medicine, Transplantation Institute, Medical University of Warsaw Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000600009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2013.04.052"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1ib00058f"},{"key":"e_1_2_8_5_1","first-page":"493","article-title":"Sodium ascorbate and basic fibroblast growth factor protect muscle\u2010derived cells from H2O2\u2010induced oxidative stress","volume":"56","author":"Burdzinska A","year":"2006","journal-title":"Comp Med"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2008.11.024"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2013.03.030"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2003.10.035"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/246134"},{"key":"e_1_2_8_10_1","first-page":"501","article-title":"Clinical evaluation of a single daily dose of phenylpropanolamine in the treatment of urethral sphincter mechanism incompetence in the bitch","volume":"52","author":"Claeys S","year":"2011","journal-title":"Can Vet J"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20998"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.10009"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368911X623835"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/CBI20100586"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02624469"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000381534"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","unstructured":"JayJ.2008.Urologic Anatomic Correlates. in: Davila GW Ghoniem GM Wexner SD editors. Plevic Floor Dysfunction. A Multidisciplinary Approach. Springer. p 71\u201379.","DOI":"10.1007\/1-84628-010-9_12"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2011.624651"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603514548213"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000317322"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2010.02471.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11365"},{"key":"e_1_2_8_23_1","first-page":"449","article-title":"Lipid rafts mediate epigallocatechin\u20103\u2010gallate\u2010 and green tea extract\u2010dependent viability of human colon adenocarcinoma COLO 205 cells; clusterin affects lipid rafts\u2010associated signaling pathways","volume":"62","author":"Pajak B","year":"2011","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2014.02.047"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.125.6.1275"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0264.2004.00575.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.02.022"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000046188"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2005.09.055"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-010-9306-9"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1590\/1677-553820133806809"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.22056"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2006.tb00703.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2014-0114"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2015.00085"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20175"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.18094"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-003-0313-x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5804"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.07.135"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.23498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.23498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:50:50Z","timestamp":1759945850000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.23498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,25]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/ar.23498"],"URL":"https:\/\/doi.org\/10.1002\/ar.23498","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2016,11,25]]}}}