{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:45:33Z","timestamp":1764783933103,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81301023"],"award-info":[{"award-number":["81301023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Triptolide (TPL) is an active compound extracted from a Chinese herbal medicine <jats:italic>tripterygium wilfordii<\/jats:italic> Hook. f. (Celastraceae), which has been used as an anti\u2010inflammatory drug for years. It also inhibits the growth and proliferation of different types of cancer cells. The inhibitory effect of TPL on angiogenesis after chemical\u2010induced corneal inflammation was studied <jats:italic>in vivo<\/jats:italic>. The effects of TPL on the proliferation, apoptosis, migration, and tube formation of rat aortic endothelial cells (RAECs) were studied <jats:italic>in vitro<\/jats:italic>. Cell proliferation and apoptosis were measured by MTT assay and flow cytometry, respectively. Migration was analyzed using the scratch wound healing assay and transwell assay. Tube formation assay was used to examine angiogenesis. Real\u2010time PCR and Western blot were used to determine the expression of vascular endothelial growth factor A (VEGFA) and VEGFC. To study the <jats:italic>in vivo<\/jats:italic> effects of TPL, the mouse model of alkali burn\u2010induced corneal angiogenesis was used. The angiogenesis was analyzed by determining the density of the newly generated blood vessels in corneas. We found that TPL induced apoptosis and inhibited the proliferation of RAECs in a dose\u2010dependent manner. TPL inhibited migration and tube formation of RAECs and decreased the expression of VEGFA and VEGFC <jats:italic>in vitro<\/jats:italic>. Furthermore, TPL suppressed alkali burn\u2010induced corneal angiogenesis and inhibited the expression of VEGFA and VEGFC in corneas <jats:italic>in vivo<\/jats:italic>. In conclusion, topical TPL as a pharmacological agent has the ability to reduce angiogenesis in cornea and may have clinical indications for the treatment of corneal angiogenesis diseases which have to be further explored. Anat Rec, 300:1348\u20131355, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23583","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T03:43:28Z","timestamp":1487907808000},"page":"1348-1355","source":"Crossref","is-referenced-by-count":8,"title":["Triptolide Suppresses Alkali Burn\u2010Induced Corneal Angiogenesis Along with a Downregulation of VEGFA and VEGFC Expression"],"prefix":"10.1002","volume":"300","author":[{"given":"Geng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anatomy Harbin Medical University Harbin 150081 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Harbin Medical University Harbin 150081 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Anatomy 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