{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T18:25:39Z","timestamp":1779301539113,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,4,17]],"date-time":"2017-04-17T00:00:00Z","timestamp":1492387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL134229"],"award-info":[{"award-number":["HL134229"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL094567"],"award-info":[{"award-number":["HL094567"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA009535"],"award-info":[{"award-number":["CA009535"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["ES000002"],"award-info":[{"award-number":["ES000002"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003006","name":"Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003006","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In the normal lung, a dominant structural element is an elastic \u201cline element\u201d that originates in the central bronchi and inserts into the distal airspaces. Despite its structural importance, the process that leads to development of the cable line element is unknown. To investigate the morphologic events contributing to its development, we used optical clearing methods to examine the postnatal rat lung. An unexpected finding was numerous spheres, with a median diameter of 1\u20132 \u00b5m, within the primary septa of the rat lung. The spheres demonstrated green autofluorescence, selective fluorescent eosin staining, reactivity with carboxyfluorescein succinimidyl ester, and specific labeling with anti\u2010tropoelastin monoclonal antibody\u2014findings consistent with tropoelastin. The sphere number peaked on rat postnatal day 4 (P4) and were rare by P14. The disappearance of the spheres was coincident with the development of the cable line element in the rat lung. Transmission electron microscopy demonstrated no consistent association between parenchymal cells and sphere alignment. In contrast, the alignment of tropoelastin spheres appeared to be the direct result of interactions of scaffold proteins including collagen fibers and fibrillin microfibrils. We conclude that the spatial organization of the cable line element appears to be independent of tropoelastin deposition, but dependent on crosslinking to scaffold proteins within the primary septa. Anat Rec, 300:1670\u20131679, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23603","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T19:33:29Z","timestamp":1491420809000},"page":"1670-1679","source":"Crossref","is-referenced-by-count":9,"title":["Extracellular Assembly of the Elastin Cable Line Element in the Developing Lung"],"prefix":"10.1002","volume":"300","author":[{"given":"Cristian D.","family":"Valenzuela","sequence":"first","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willi L.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D.","family":"Bennett","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra B.","family":"Ysasi","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janeil M.","family":"Belle","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Molter","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beate K.","family":"Straub","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering Dartmouth College Hanover NH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi","family":"Chen","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering Dartmouth College Hanover NH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maximilian","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Tsuda","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Physiological Sciences, Harvard School of Public Health Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Mentzer","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-57-1-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/erm.2013.9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091780405"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.81.4.1800"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2007.08.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2014.05.014"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0610092"},{"key":"e_1_2_7_9_1","first-page":"235","article-title":"Observations sur le developpement du poumon humain","volume":"13","author":"Dubreuil G","year":"1936","journal-title":"Bull Histol Appl Tech Microsc"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"15115","DOI":"10.1016\/S0021-9258(18)63819-0","article-title":"Role of tropoelastin fragmentation in elastogenesis in rat smooth\u2010muscle cells","volume":"264","author":"Franzblau C","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01081407"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.23237"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1991.tb00881.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2928"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199308000-00008"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.5021\/ad.2011.23.1.44"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008208709006976"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2011.0584"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/5.3.206"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2001.1027"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20647"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.20.3943"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.01.055"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1997.272.3.L452"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78247-X"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-8165(73)80016-2"},{"key":"e_1_2_7_27_1","first-page":"91","volume-title":"Fishman's pulmonary diseases and disorders","author":"Schittny JC","year":"2008"},{"key":"e_1_2_7_28_1","first-page":"69","article-title":"Sirius red F3BA as stain for connective tissue","volume":"78","author":"Sweat F","year":"1964","journal-title":"Arch Pathol"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.34.3.338"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22649"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01355.2013"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2009.11.003"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23259"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.02.062"},{"key":"e_1_2_7_35_1","first-page":"89","volume-title":"Mechanics of Breathing, part 1, Handbook of physiology, sec 3: The Respiratory System. Bethesda, Md., American Physiological Society","author":"Weibel ER.","year":"1986"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01203.2012"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.23.3.3906"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1982.52.4.1064"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.03.017"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.23603","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.23603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:55:59Z","timestamp":1759974959000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.23603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,17]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/ar.23603"],"URL":"https:\/\/doi.org\/10.1002\/ar.23603","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,17]]}}}