{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:59:05Z","timestamp":1759971545176,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"KAKENHI (Grant-in-Aid for Scientific Research (C)","award":["17K01523"],"award-info":[{"award-number":["17K01523"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The external urethral sphincter is a unique striated muscle surrounding the urethra that plays a crucial role in urinary continence, and a comprehensive understanding of its morphology is needed to determine the pathophysiology underlying urinary incontinence and find suitable therapies. Differences between the sexes and among species regarding the fiber types present remain controversial. This study used triple immunofluorescence labeling to visualize one slow (Type 1) and two fast (Types 2A and 2B) myosin isoforms in rat external urethral sphincters from both sexes. Type 2A fibers predominated throughout the sphincter and Type 2B fibers were restricted to the proximal one\u2010third of the external urethral sphincter in the female rats. Type 1 fibers were present adluminally and were concentrated in the proximal and distal segments of the sphincter. While most of the male external urethral sphincter comprised Type 2B fibers, Type 2A fibers intermingled among these fibers in the proximal one\u2010third of the sphincter, and a few Type 1 fibers were present that were restricted to the adluminal region of the proximal segment. The fiber\u2010type compositions and their areal densities changed in both sexes after gonadectomy. The areal density of the Type 1 fibers increased significantly in the ovariectomized females, especially in the distal segment. In the orchidectomized males, the areal densities of the Types 1 and 2A fibers increased significantly, but that of the Type 2B fibers decreased. These results indicate that myosin heavy chain expression in the rat external urethral sphincter is sexually dimorphic and shows regional differences. Anat Rec, 2017. \u00a9 2017 Wiley Periodicals, Inc. 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