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Seventy percent of the vault's mass consists of a \u2248100 kDa protein, the major vault protein (MVP). In humans, a drug resistance\u2010associated protein, originally identified as lung resistance protein in metastatic lung cancer, was ultimately shown to be the previously described MVP. In this study, a partial MVP sequence was cloned from channel catfish. Recombinant MVP (rMVP) was used to generate a monoclonal antibody that recognizes full length protein in distantly related fish species, as well as mice. MVP is expressed in fish spleen, liver, anterior kidney, renal kidney, and gills, with a consistent expression in epithelial cells, macrophages, or endothelium at the interface of the tissue and environment or vasculature. We show that vaults are distributed throughout cells of fish lymphoid cells, with nuclear and plasma membrane aggregations in some cells. Protein expression studies were extended to liver neoplastic lesions in Atlantic killifish collected in situ at the Atlantic Wood USA\u2010EPA superfund site on the southern branch of the Elizabeth River, VA. MVP is highly expressed in these lesions, with intense staining at the nuclear membrane, similar to what is known about MVP expression in human liver neoplasia. Additionally, MVP mRNA expression was quantified in channel catfish ovarian cell line following treatment with different classes of pharmacological agents. Notably, mRNA expression is induced by ethidium bromide, which damages DNA. Anat Rec, 2017. \u00a9 2017 Wiley Periodicals, Inc. Anat Rec, 300:1981\u20131992, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23645","type":"journal-article","created":{"date-parts":[[2017,7,15]],"date-time":"2017-07-15T04:24:53Z","timestamp":1500092693000},"page":"1981-1992","source":"Crossref","is-referenced-by-count":12,"title":["Expression of the Major Vault Protein (MVP) and Cellular Vault Particles in Fish"],"prefix":"10.1002","volume":"300","author":[{"given":"Alyssa L.","family":"Margiotta","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Clemson University Clemson South Carolina 29634"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa J.","family":"Bain","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Clemson University Clemson South Carolina 29634"},{"name":"Environmental Toxicology Graduate Program Clemson University Clemson South Carolina 29634"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5352-9165","authenticated-orcid":false,"given":"Charles D.","family":"Rice","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Clemson University Clemson South Carolina 29634"},{"name":"Environmental Toxicology Graduate Program Clemson University Clemson South Carolina 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