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Two weeks post orchidectomy (Orx), animals received estradiol\u2010dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham\u2010operated (So) and Orx rats received just the vehicle. Changes in the volumes of <jats:italic>pars distalis<\/jats:italic>, of individual follicle\u2010stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design\u2010based stereology. The intracellular content of \u03b2FSH and \u03b2LH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of <jats:italic>pars distalis<\/jats:italic>, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol\u2010like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of <jats:italic>pars distalis<\/jats:italic>, decreased the volume density of LH\u2010labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH\u2010containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx\u2010induced changes in pituitary castration cells. Anat Rec, 2018. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23809","type":"journal-article","created":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T06:26:12Z","timestamp":1521786372000},"page":"1416-1425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study"],"prefix":"10.1002","volume":"301","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6218-988X","authenticated-orcid":false,"given":"Nata\u0161a","family":"Nestorovi\u0107","sequence":"first","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Trifunovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milica","family":"Manojlovi\u0107\u2010Stojanoski","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Jari\u0107","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nata\u0161a","family":"Risti\u0107","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Branko","family":"Filipovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Branka","family":"\u0160o\u0161i\u0107\u2010Jurjevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verica","family":"Milo\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Department of Cytology University of Belgrade, Institute for Biological Research \u201cSini\u0161a Stankovi\u0107,\u201d Belgrade 11060 Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,30]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"13","article-title":"Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle\u2010aged male rats","volume":"42","author":"Ajd\u017eanovi\u0107 V","year":"2009","journal-title":"Biol Res"},{"doi-asserted-by":"publisher","key":"e_1_2_6_3_1","DOI":"10.3181\/0807-RM-231"},{"key":"e_1_2_6_4_1","first-page":"1257","article-title":"Daidzein effects on ACTH cells: Immunohistomorphometric and hormonal study in an animal model of the andropause","volume":"26","author":"Ajd\u017eanovi\u0107 V","year":"2011","journal-title":"Histol Histopathol"},{"doi-asserted-by":"publisher","key":"e_1_2_6_5_1","DOI":"10.1530\/JOE-13-0158"},{"doi-asserted-by":"publisher","key":"e_1_2_6_6_1","DOI":"10.1679\/aohc.74.41"},{"doi-asserted-by":"publisher","key":"e_1_2_6_7_1","DOI":"10.1016\/S0301-2115(98)00281-4"},{"doi-asserted-by":"publisher","key":"e_1_2_6_8_1","DOI":"10.1016\/B978-012515400-0\/50034-8"},{"key":"e_1_2_6_9_1","first-page":"1","article-title":"Role of estrogen receptors and G protein\u2010coupled estrogen receptor in regulation of hypothalamus\u2010pituitary\u2010testis axis and spermatogenesis","volume":"16","author":"Chimento A","year":"2014","journal-title":"Front Endocrinol (Lausanne)"},{"doi-asserted-by":"publisher","key":"e_1_2_6_10_1","DOI":"10.1289\/ehp.02110s3349"},{"doi-asserted-by":"publisher","key":"e_1_2_6_11_1","DOI":"10.1046\/j.1365-2818.2001.00958.x"},{"doi-asserted-by":"publisher","key":"e_1_2_6_12_1","DOI":"10.1210\/endo-54-5-516"},{"doi-asserted-by":"publisher","key":"e_1_2_6_13_1","DOI":"10.1056\/NEJMra000471"},{"doi-asserted-by":"publisher","key":"e_1_2_6_14_1","DOI":"10.1111\/j.1365-2818.1987.tb02837.x"},{"volume-title":"Unbiased stereology. 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