{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:27:49Z","timestamp":1778002069559,"version":"3.51.4"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T00:00:00Z","timestamp":1525651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The presence of estrogen in the genital ducts of different mammalian species has been extensively studied and the estrogen influence on the functional activity of the male genital tract has been hypothesized. Conversely, very few data have been reported on pig excurrent ducts: the localization of classical estrogen receptors (ER\u03b1 and ER\u03b2) is scarcely known, while the expression of the G protein\u2010coupled receptor (GPER1), a membrane estrogen receptor, is still unknown in pig. The aim of the present study was to evaluate GPER1 expression in the different regions of the mature pig epididymis, using immunohistochemistry, western blot and RT\u2010PCR analyses. The results showed that GPER1 is mainly expressed in the epithelial cells of the corpus epididymis compared to the caput and the cauda, while muscle cells are moderately immunostained and stromal cells are unstained. The presence of GPER1 was confirmed by Western blot and RT\u2010PCR analyses. In our study, we have demonstrated for the first time the GPER1 expression in male porcine epididymis, revealing a new mediator of estrogen signaling at this site. In conclusion, these new data suggest that estrogen action via GPER1 may contribute to sperm maturation in the corpus and sperm protection\/storage in the cauda. Interestingly, the presence of GPER1 in the muscle layer may be indicative of a possible GPER1 involvement in the estrogen regulation of duct contractility. Anat Rec, 2018. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.23837","type":"journal-article","created":{"date-parts":[[2018,4,20]],"date-time":"2018-04-20T22:48:26Z","timestamp":1524264506000},"page":"1467-1473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Immunolocalization of G Protein\u2010Coupled Estrogen Receptor in the Pig Epididymis"],"prefix":"10.1002","volume":"301","author":[{"given":"Rocco","family":"Malivindi","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences University of Calabria Arcavacata di Rende Cosenza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saveria","family":"Aquila","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences University of Calabria Arcavacata di Rende Cosenza Italy"},{"name":"Centro Sanitario University of Calabria Arcavacata di 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