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Current therapies use microsurgical approaches but there is the potential for enhancing recovery through other therapeutic modalities such as; cell\u2010based conduits, gene therapy and small molecules. A number of molecular targets and drugs which have the potential to improve nerve regeneration have been identified, however, there are challenges associated with moving therapies toward clinical translation. Due to the lack of detailed knowledge about the pro\u2010regenerative effect of potential drug treatments, there is a need for effective <jats:italic>in vitro<\/jats:italic> models to screen compounds to inform future pre\u2010clinical and clinical studies. The interaction between regenerating neurites and supporting Schwann cells is a key feature of the nerve environment, therefore, <jats:italic>in vitro<\/jats:italic> models that mimic this cellular association are useful tools. In this study, we have investigated various cell culture models, including simple monolayer systems and more complex 3D\u2010engineered co\u2010cultures, as models for use in PNI drug development. Anat Rec, 301:1628\u20131637, 2018. \u00a9 2018 The Authors. <jats:italic>The Anatomical Record<\/jats:italic> published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.<\/jats:p>","DOI":"10.1002\/ar.23918","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T01:19:59Z","timestamp":1539825599000},"page":"1628-1637","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Developing an <i>In Vitro<\/i> Model to Screen Drugs for Nerve Regeneration"],"prefix":"10.1002","volume":"301","author":[{"given":"MELISSA L. 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