{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:08:22Z","timestamp":1790687302431,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,11,22]],"date-time":"2018-11-22T00:00:00Z","timestamp":1542844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"The mechanism of glutamatergic neurons in the lateral habenula nucleus involved in the control of chronic pain and depression","award":["National Natural Science Foundation of China (No. 81571074)"],"award-info":[{"award-number":["National Natural Science Foundation of China (No. 81571074)"]}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Lateral parabrachial nucleus (LPB) is a critical region in the integration and transmission of peripheral nociceptive information. The parabrachio\u2010amygdaloid (P\u2010Amy) pathway and parabrachio\u2010ventral tegmental area (P\u2010VTA) pathway is thought to be significant in regulation of pain\u2010related negative emotions. In present study, retrograde tract tracers Fluoro\u2010gold (FG) and tetramethylrhodramine\u2010dextran (TMR) were stereotaxically injected into the right central amygdaloid nucleus (CeA) and right VTA, respectively. Then, part of these rats were performed with the spare nerve injury (SNI) in the controlateral side of FG and TMR injection. Afterwards, double\u2010 or triple\u2010immunofluorescent histochemistry was used to examine FG\/TMR double\u2010 and FG\/TMR\/FOS or FG\/TMR\/CGRP triple\u2010labeled neurons in the LPB. The results showed that all of FG, TMR single\u2010 and FG\/TMR double\u2010labeled neurons were distributed in the LPB bilaterally with an ipsilateral predominance. The proportion of FG\/TMR double\u2010labeled neurons to the total number of FG\u2010 and TMR\u2010labeled neurons was 10.78% and 13.07%, respectively. Nearly all of the FG\/TMR double\u2010labeled neurons (92.67%) showed calcitonin gene\u2010related peptide (CGRP) immunopositive. On the other hand, in the SNI rats, about 89.49% and 77.87% of FG\u2010 and TMR\u2010labeled neurons were FG\/FOS\u2010 and TMR\/FOS\u2010positive neurons; about 93.33% of the FG\/TMR double\u2010labeled neurons were FOS\u2010LI. Our results suggest that the part of CGRP immunopositive neurons in the LPB send projection fibers to both the CeA and VTA by the way of axon collaterals, which are activated by the nociceptive stimulation in the SNI condition, and may play an important role in the transmission of peripheral nociceptive information. Anat Rec, 302:1178\u20131186, 2019. \u00a9 2018 The Authors. <jats:italic>The Anatomical Record<\/jats:italic> published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.<\/jats:p>","DOI":"10.1002\/ar.23983","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T18:24:26Z","timestamp":1539800666000},"page":"1178-1186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Collateral Projections from the Lateral Parabrachial Nucleus to the Central Amygdaloid Nucleus and the Ventral Tegmental Area in the Rat"],"prefix":"10.1002","volume":"302","author":[{"given":"Yu","family":"Qiao","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology and Embryology and K. K. Leung Brain Research Centre The Fourth Military Medical University  Xi'an, 710032 China"},{"name":"Student Brigade The Fourth Military Medical University  Xi'an, 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Kui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology and Embryology and K. K. Leung Brain Research Centre The Fourth Military Medical University  Xi'an, 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology and Embryology and K. K. Leung Brain Research Centre The Fourth Military Medical University  Xi'an, 710032 China"},{"name":"Department of Neurosurgery Tangdu Hospital, The Fourth Military Medical University  Xi'an, 710038 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze\u2010Hao","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology and Embryology and K. K. Leung Brain Research Centre The Fourth Military Medical University  Xi'an, 710032 China"},{"name":"Student Brigade The Fourth Military Medical University  Xi'an, 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology and Embryology and K. K. Leung Brain Research Centre The Fourth Military Medical University  Xi'an, 710032 China"},{"name":"Student Brigade The Fourth Military Medical University  Xi'an, 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Lian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology and Embryology and K. K. 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