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To achieve a higher efficiency and shorter turn\u2010around time for NGS library preparation, here, we compared NGS library preparation processes and outcomes with three commercial library construction methods and two hybridization capture methods thus, developed an improved NGS library construction approach. This improved approach took advantage of both methods and resulted in a higher output from the same input DNA, including higher library construction success rate, higher probe capture rate, and shorter turn\u2010around time. Using this approach, targeted region libraries could be constructed within only 1 day for FFPE samples; therefore, this approach has potential applications of NGS in routine clinical tests. Anat Rec, 302:941\u2013946, 2019. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/ar.24002","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T11:19:15Z","timestamp":1540552755000},"page":"941-946","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An Improved NGS Library Construction Approach Using DNA Isolated from Human Cancer Formalin\u2010Fixed Paraffin\u2010Embedded Samples"],"prefix":"10.1002","volume":"302","author":[{"given":"Chunze","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin, 300121 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery Tianjin Union Medical Center  Tianjin, 300121 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