{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:07:15Z","timestamp":1787803635009,"version":"build-2784847793"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T00:00:00Z","timestamp":1553644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Natural Science Foundation of Fujian Provincial Department of Science & Technology","award":["2017J01530"],"award-info":[{"award-number":["2017J01530"]}]},{"name":"Science and Technology Innovation Joint Fund Project of Fujian Provincial Department of Science & Technology","award":["2017Y9019"],"award-info":[{"award-number":["2017Y9019"]}]},{"name":"Science and Technology Plan Project of Fujian Provincial Department of Science & Technology","award":["2018Y0039"],"award-info":[{"award-number":["2018Y0039"]}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Melatonin, a neurohormone secreted by the pineal gland, has a variety of biological functions, such as circadian rhythms regulation, anti\u2010oxidative activity, immunomodulatory effects, and anittumor, etc. At present, its antitumor effect has attracted people's attention due to its extensive tissue distribution, good tissue compatibility, and low toxic and side effects. In the gastrointestinal tract, there is high level of melatonin and many studies showed melatonin has effects of anti\u2010gastric cancer. In this experiment, human gastric cancer cell lines AGS and MGC803 were used to investigate the intracellular molecular mechanism of melatonin against gastric cancer. After AGS and MGC803 have been treated with melatonin, the changes of cell morphology and cellular structure were observed under electron microscope. Flow cytometer and apoptosis detection kits were used to analyze the effect of apoptosis on AGS and MGC803. The alterations of apoptosis\u2010related proteins Caspase 9, Caspase 3, and upstream regulators AKT, MDM2 including expression, phosphorylation, and activation were detected to analyze the intracellular molecular mechanism of melatonin inhibiting gastric cancer. In AGS and MGC803 cells with melatonin exposure, cleaved Caspase 9 was upregulated and Caspase 3 was activated; moreover, MDM2 and AKT expression and phosphorylation were downregulated. Melatonin promoted apoptosis of AGS and MGC803 cells by the downregulation of AKT and MDM2. Anat Rec, 302:1544\u20131551, 2019. \u00a9 2019 American Association for Anatomy<\/jats:p>","DOI":"10.1002\/ar.24101","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T03:48:29Z","timestamp":1551239309000},"page":"1544-1551","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Downregulation of AKT and MDM2, Melatonin Induces Apoptosis in AGS and MGC803 Cells"],"prefix":"10.1002","volume":"302","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9447-2273","authenticated-orcid":false,"given":"Jun","family":"Song","sequence":"first","affiliation":[{"name":"Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian, 350122 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai\u2010Jun","family":"Ma","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Second Inpatient Department Fuzhou General Hospital  Fuzhou Fujian, 350108 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Hua","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian, 350122 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian, 350122 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian, 350122 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Guang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian, 350122 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u2010Shan","family":"Chen","sequence":"additional","affiliation":[{"name":"Pathology The Affiliated Union Hospital of Fujian Medical University  Fuzhou Fujian, 350001 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Xiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian, 350122 People's Republic of 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