{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T22:34:02Z","timestamp":1776897242432,"version":"3.51.2"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,6,2]],"date-time":"2019-06-02T00:00:00Z","timestamp":1559433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["NO. 2014A020212025"],"award-info":[{"award-number":["NO. 2014A020212025"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>miR\u2010455\u20105p and retinoid signaling pathway and its membrane receptor, <jats:italic>STRA6<\/jats:italic>, are associated with lung development. Software copredictions indicate that the miRNA upstream of the <jats:italic>STRA6<\/jats:italic> gene is miR\u2010455\u20105p. We hypothesized that miR\u2010455\u20105p participates in rat lung alveolar Type II cell proliferation by targeting <jats:italic>STRA6<\/jats:italic> and designed this study to investigate the effects of miR\u2010455\u20105p overexpression on rat lung alveolar Type II cells. Dual luciferase reporter gene assay was utilized to confirm the relationship between miR\u2010455\u20105p and <jats:italic>STRA6<\/jats:italic>. An miR\u2010455\u20105p\u2010expressing adenoviral vector was constructed and transfected into rat lung alveolar Type II cells. <jats:italic>STRA6<\/jats:italic> protein expression was detected in rat lung alveolar Type II cells by Western blotting at 72\u2009hr posttransfection. Retinol concentration was detected by ELISA at 72\u2009hr posttransfection. The cell proliferation was detected by CCK8 assay at 24, 48, and 72\u2009hr posttransfection. Our results showed that <jats:italic>STRA6<\/jats:italic> is a target gene of miR\u2010455\u20105p. <jats:italic>STRA6<\/jats:italic> protein expression was significantly lower in the miR\u2010455\u20105p\u2010overexpression group than in the NC group (0.615\u2009\u00b1\u20090.131 vs. 0.958\u2009\u00b1\u20090.246, <jats:italic>P<\/jats:italic> = 0.029). Similar results were observed for retinol concentration (2.985\u2009\u00b1\u20090.061 vs. 3.949\u2009\u00b1\u20090.118, <jats:italic>P<\/jats:italic> = 0.000). Rat lung alveolar Type II cell proliferation was lower in the miR\u2010455\u20105p\u2010overexpression group than in the NC group at 24, 48, and 72\u2009hr posttransfection (24\u2009hr: 0.280\u2009\u00b1\u20090.184 vs. 1.354\u2009\u00b1\u20090.169 <jats:italic>P<\/jats:italic> = 0.026; 48\u2009hr: 0.881\u2009\u00b1\u20090.016 vs. 1.992\u2009\u00b1\u20090.050 <jats:italic>P<\/jats:italic> = 0.001; 72\u2009hr: 2.105\u2009\u00b1\u20090.148 vs. 2.937\u2009\u00b1\u20090.079 <jats:italic>P<\/jats:italic> = 0.016). In summary, miR\u2010455\u20105p is associated with lung development. miR\u2010455\u20105p overexpression downregulates <jats:italic>STRA6<\/jats:italic>, leading to reduced retinol concentration and rat lung alveolar Type II cell proliferation. Anat Rec, 302:2062\u20132069, 2019. \u00a9 2019 The Authors. <jats:italic>The Anatomical Record<\/jats:italic> published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.<\/jats:p>","DOI":"10.1002\/ar.24145","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T12:42:50Z","timestamp":1557837770000},"page":"2062-2069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["miR\u2010455\u20105p Overexpression Reduces Rat Lung Alveolar Type II Cell Proliferation by Downregulating <i>STRA6<\/i>"],"prefix":"10.1002","volume":"302","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0667-0067","authenticated-orcid":false,"given":"Jintao","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Foshan Maternity and Children's Healthcare Hospital Affiliated to Southern Medical University  Guangzhou Guangdong China"},{"name":"Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuming","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huajian","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Foshan Maternity and Children's Healthcare Hospital Affiliated to Southern Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Foshan Maternity and Children's Healthcare Hospital Affiliated to Southern Medical University  Guangzhou Guangdong China"},{"name":"Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0010010"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63861-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.06.020"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.2012.01904.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI70291"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.22.010402.102745E"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010854"},{"key":"e_1_2_6_10_1","first-page":"L705","article-title":"Retinoids control surfactant phospholipid biosynthesis in fetal rat 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