{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:00:39Z","timestamp":1787068839176,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Non\u2010small\u2010cell lung cancer (NSCLC) is one of the most common human malignancies. Amentoflavone (AF) is one of bioflavonoid compounds isolated from Selaginella tamariscina Spring. This study was designed to examine the effect of AF on NSCLC. Our results indicated that AF decreased cell viability of both H1299 and H358 cells. Colony formation assay also showed that AF was able to suppress the anchorage\u2010independent growth of NSCLC cells. AF also triggered cell cycle arrest by downregulating cyclin D1, CDK4, and CDK6. The pro\u2010apoptotic activity of AF was confirmed by Hoechst staining and flow cytometry. The effect of AF on activation of caspase\u20103, upregulation of Bax, and downregulation of Bcl\u20102 was examined by western blot. The anti\u2010growth and pro\u2010apoptotic activities of AF were further validated in xenograft murine model. iTRAQ assay showed that cancerous inhibitor of PP2A (CIP2A) expression was markedly downregulated by AF treatment in H1299 cells. In addition, qRT\u2010PCR and western blot also showed that AF was able to dose\u2010dependently inhibit CIP2A expression. Meanwhile, the activity of protein phosphatase 2A (PP2A) was enhanced by AF treatment. The mRNA and protein expression of CIP2A as well as PP2A activity in xenograft tumor tissue were examined, which indicated that the\n                    <jats:italic>in vivo<\/jats:italic>\n                    anticancer activity of AF was associated with downregulation of CIP2A and reactivation of PP2A. Moreover, our results showed that the anti\u2010growth and pro\u2010apoptotic activities of AF were augmented by CIP2A knockdown and attenuated by ectopic CIP2A expression. Our results indicated that AF exhibited anticancer activity in NSCLC by targeting CIP2A. Anat Rec, 302:2201\u20132210, 2019. \u00a9 2019 American Association for Anatomy\n                  <\/jats:p>","DOI":"10.1002\/ar.24229","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T11:54:21Z","timestamp":1566388461000},"page":"2201-2210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<i>Retracted<\/i>\n                    : Amentoflavone Promotes Apoptosis in Non\u2010Small\u2010Cell Lung Cancer by Modulating Cancerous Inhibitor of PP2A"],"prefix":"10.1002","volume":"302","author":[{"given":"Fei","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5816-5535","authenticated-orcid":false,"given":"Yijiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1054\/plef.2002.0387"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.357"},{"key":"e_1_2_6_4_1","first-page":"6685","article-title":"Amentoflavone induces anti\u2010angiogenic and anti\u2010metastatic effects through suppression of NF\u2010kappaB activation in MCF\u20107 cells","volume":"35","author":"Chen JH","year":"2015","journal-title":"Anticancer Res"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-3283"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1350672"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2173"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2010.0053"},{"key":"e_1_2_6_9_1","first-page":"2565","article-title":"Inhibition of miR\u2010141 reverses cisplatin resistance in non\u2010small cell lung cancer cells via upregulation of programmed cell death protein 4","volume":"20","author":"Fu WF","year":"2016","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-981562"},{"key":"e_1_2_6_11_1","first-page":"2445","article-title":"CIP2A depletion potentiates the chemosensitivity of cisplatin by inducing increased apoptosis in bladder cancer cells","volume":"40","author":"Gao F","year":"2018","journal-title":"Oncol Rep"},{"key":"e_1_2_6_12_1","first-page":"285","article-title":"Amentoflavone, a biflavonoid from Biophytum sensitivum augments lymphocyte proliferation, natural killer cell and antibody dependent cellular cytotoxicity through enhanced production of IL\u20102 and IFN\u2010gamma and restrains serum sialic acid and gamma glutamyl transpeptidase production in tumor \u2010 bearing animals","volume":"6","author":"Guruvayoorappan C","year":"2007","journal-title":"J Exp Ther Oncol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1534735407302345"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/08923970802278276"},{"key":"e_1_2_6_15_1","first-page":"207","article-title":"Amentoflavone stimulates apoptosis in B16F\u201010 melanoma cells by regulating bcl\u20102, p53 as well as caspase\u20103 genes and regulates the nitric oxide as well as proinflammatory cytokine production in B16F\u201010 melanoma cells, tumor associated macrophages and peritoneal macrophages","volume":"7","author":"Guruvayoorappan C","year":"2008","journal-title":"J Exp Ther Oncol"},{"key":"e_1_2_6_16_1","first-page":"209","article-title":"Inhibition of tumor specific angiogenesis by amentoflavone","volume":"73","author":"Guruvayoorappan C","year":"2008","journal-title":"Biochemistry"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.09.012"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.56.2.106"},{"key":"e_1_2_6_19_1","first-page":"8343","article-title":"Long non\u2010coding RNA UCA1 regulates the proliferation, migration and invasion of human lung cancer cells by modulating the expression of microRNA\u2010143","volume":"22","author":"Jun T","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.03.010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.04.044"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.32.1427"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2010.1428"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4778"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.11351"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4137"},{"key":"e_1_2_6_27_1","first-page":"1211","article-title":"Cancerous inhibitor of protein phosphatase 2A regulates cisplatin resistance in ovarian cancer","volume":"17","author":"Li W","year":"2019","journal-title":"Oncol Lett"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-1012-z"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.07.177"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.09.026"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13575"},{"key":"e_1_2_6_32_1","first-page":"8722","article-title":"LncRNA HULC promotes non\u2010small cell lung cancer cell proliferation and inhibits the apoptosis by up\u2010regulating sphingosine kinase 1 (SPHK1) and its downstream PI3K\/Akt pathway","volume":"22","author":"Liu L","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_6_33_1","first-page":"1279","article-title":"Polyphyllin I inhibits invasion and epithelial\u2010mesenchymal transition via CIP2A\/PP2A\/ERK signaling in prostate cancer","volume":"53","author":"Liu X","year":"2018","journal-title":"Int J Oncol"},{"key":"e_1_2_6_34_1","first-page":"963","article-title":"Amentoflavone induces cell\u2010cycle arrest and apoptosis in MCF\u20107 human breast cancer cells via mitochondria\u2010dependent pathway","volume":"26","author":"Pei JS","year":"2012","journal-title":"In Vivo"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3012"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-013-1539-y"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-014-1596-4"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.08.052"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.10.013"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177123"},{"key":"e_1_2_6_42_1","first-page":"8731","article-title":"Elevated PHD2 expression might serve as a valuable biomarker of poor prognosis in lung adenocarcinoma, but no lung squamous cell carcinoma","volume":"22","author":"Xu XL","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_6_43_1","first-page":"1035","article-title":"Amentoflavone induces apoptosis in SW480 human colorectal cancer cells via regulating beta\u2010catenin and caspase\u20103 expressions","volume":"34","author":"Yang Y","year":"2014","journal-title":"J South Med Univ"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1298175"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2018.07.008"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2018.08.032"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.10.028"}],"updated-by":[{"DOI":"10.1002\/ar.24489","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T00:00:00Z","timestamp":1594857600000},"record-id":"24419"},{"DOI":"10.1002\/ar.24489","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T00:00:00Z","timestamp":1594857600000}}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24229","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.24229","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:40:08Z","timestamp":1759930808000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.24229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,2]]},"references-count":46,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/ar.24229"],"URL":"https:\/\/doi.org\/10.1002\/ar.24229","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,2]]},"assertion":[{"value":"2019-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}