{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T23:59:45Z","timestamp":1769731185188,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T00:00:00Z","timestamp":1578441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Factors influencing the damage to the inner ear can include the surgical approach used for vector delivery, the volume of vector used, the buffer that the vector is suspended in as well as the host response to the vector capsid and vector genes that are transferred. We evaluated the effect of Ad5 capsid adenovectors on hearing function after delivery to the perilymph of adult C57Bl\/6 mice. Hearing was evaluated before surgery and 3\u2009days post\u2010surgery by auditory brain stem response (ABR) and distortion product otoacoustic emissions (DPOAE). A second group of mice underwent repeated delivery of adenovector two times to determine if a preliminary exposure to an Ad vector could induce an inflammatory response leading to further loss. The first adenovector (Ad.11D.LacZ) was delivered to the posterior semicircular canal or <jats:italic>via<\/jats:italic> round window. In the second surgery, a second adenovector (Ad.11D.gfp) was delivered to the horizontal semicircular canal. The functional outcome was tested prior, 7\u2009days post first vector delivery, and 3\u2009days post second vector delivery <jats:italic>via<\/jats:italic> ABR and DPOAE. Dual delivery <jats:italic>via<\/jats:italic> the semicircular canals resulted in preservation of hearing suggesting that pre\u2010exposure to Ad5 capsid does not predispose to hearing loss. Delivery to the round window resulted in hearing loss that was worsened after the second vector delivery, suggesting that delivery route and prior injury to the inner ear rather than the repetition of delivery predisposes to further hearing loss.  Anat Rec, 303:600\u2013607, 2020. \u00a9 2019 American Association for Anatomy<\/jats:p>","DOI":"10.1002\/ar.24347","type":"journal-article","created":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T23:50:13Z","timestamp":1578527413000},"page":"600-607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Hearing Preservation Following Repeated Adenovector Delivery"],"prefix":"10.1002","volume":"303","author":[{"given":"Susanna C.","family":"Pfannenstiel","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery University of Kansas School of Medicine  Kansas City Kansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Praetorius","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery University of Kansas School of Medicine  Kansas City Kansas"},{"name":"Department of Otolaryngology University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas E.","family":"Brough","sequence":"additional","affiliation":[{"name":"Precigen Inc.  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