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It is a challenge to restore SGN due to the permanent degeneration and viability of SGN is requisite for patients to receive an advantage from hearing aid devices. Human dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) are self\u2010renewing stem cells that originate from the neural crest during development. These stem cells have a high potential for neuronal differentiation. This is primarily due to their multilineage differentiation potential and their relative ease of access. Previously, we have shown the ability of these stem cell types to differentiate into spiral ganglion neuron\u2010like cells. In this study, we induced the cells into neural precursor cells (NPC) and cocultured with auditory brainstem slice (ABS) encompassing cochlear nucleus by the Stoppini method. We also investigated their ability to differentiate after 2\u2009weeks and 4\u2009weeks in coculture. Neuronal differentiation of DPSC\u2010NPC and SHED\u2010NPC was higher expression of specific markers to SGN, TrkB, and Gata3, compared to monoculture. The cells also highly expressed synaptic vesicle protein (SV2A) and exhibited intracellular calcium oscillations. Our findings demonstrated the possibility of using DPSCs and SHEDs as an autologous stem cell\u2010based therapy for sensorineural hearing loss patients.<\/jats:p>","DOI":"10.1002\/ar.24368","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:42:41Z","timestamp":1578876161000},"page":"2931-2946","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Neuronal differentiation of dental pulp stem cells from human permanent and deciduous teeth following coculture with rat auditory brainstem slices"],"prefix":"10.1002","volume":"303","author":[{"given":"Thanasup","family":"Gonmanee","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Science Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hathaitip","family":"Sritanaudomchai","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of 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