{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:30:39Z","timestamp":1777519839576,"version":"3.51.4"},"reference-count":69,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T00:00:00Z","timestamp":1592438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The viscacha (<jats:italic>Lagostomus maximus<\/jats:italic>) is a rodent of nocturnal habits, whose physiology and behavior vary according to modifications of environmental signals. The objective of this study is to assess the influence of melatonin and sexual hormones on the viscacha adrenal cortex proliferative activity through the immunohistochemical detection of proliferating cell nuclear antigen (PCNA) along with hormonal determinations. PCNA expression was studied in male viscachas to assess the effect of melatonin administration, castration, and the annual reproductive cycle. In female viscachas, PCNA was studied in nonpregnant and pregnant viscachas. PCNA expression was observed in adrenocortical cells (PCNA\u2010A) and endothelial cells (PCNA\u2010E). Melatonin\u2010administered animals showed a significantly lower number of PCNA\u2010A compared to the control group. No significant difference could be established in the number of PCNA\u2010A and PCNA\u2010E between castrated and control animals. However, the morphometric analysis showed an increase in the size of the cortex of castrated animals, along with other cytological features. Significant differences in serum testosterone levels were observed during the male viscacha reproductive cycle, with the lowest levels encountered during the regression period (winter). Male viscachas exhibited a significantly high number of PCNA\u2010A during late autumn and a high number of PCNA\u2010E during winter. In females, hormonal determinations showed a peak of progesterone and estrogen during mid\u2010pregnancy, along with a notably high number of PCNA\u2010A and an increase in the number of PCNA\u2010E. Our results suggest that proliferation in the adrenal cortex of the viscacha varies in relation to melatonin, sexual hormones, and environmental conditions.<\/jats:p>","DOI":"10.1002\/ar.24457","type":"journal-article","created":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T09:50:51Z","timestamp":1590227451000},"page":"3052-3067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Influence of melatonin and sexual hormones on the expression of proliferating cell nuclear antigen in the adrenal cortex of a seasonal breeder (<scp><i>Lagostomus maximus<\/i><\/scp>)"],"prefix":"10.1002","volume":"303","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9234-2686","authenticated-orcid":false,"given":"Luis Ezequiel","family":"Gallol","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Histolog\u00eda, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis  San Luis 5700 Argentina"},{"name":"Centro Cient\u00edfico Tecnol\u00f3gico del Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  San Luis 5700 Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabricio Iv\u00e1n","family":"Busolini","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Histolog\u00eda, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis  San Luis 5700 Argentina"},{"name":"Centro Cient\u00edfico Tecnol\u00f3gico del Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  San Luis 5700 Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabian Heber","family":"Mohamed","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Histolog\u00eda, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis  San Luis 5700 Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20144"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2014.05001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00443.2012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2016.49.9.141"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(92)90033-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-014-0859-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022011000400013"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/10253890.2010.482628"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23621"},{"issue":"11","key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.4067\/S0034-98872008001100004","article-title":"Melatonin reduces cortisol response to ACTH in humans","volume":"136","author":"Campino C.","year":"2008","journal-title":"Revista M\u00e9dica de Chile"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1271693"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081865"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23261"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.5402\/2013\/128921"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1677\/ERC-07-0081"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1987.tb00858.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.002832"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009422"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-018-1798-x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1807-59322010000300009"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD13126"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2005.11.012"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2010.08.001"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod45.3.493"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2018.03.009"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.4103\/1463-1741.144424"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.30.6.1525"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.20124"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20556"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1938.sp003565"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(94)90018-3"},{"key":"e_1_2_7_33_1","first-page":"163","volume-title":"Pathobiology of Marine Mammal Diseases","author":"Howard E. B.","year":"1983"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2152\/jmi.51.202"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2004.00119.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/837901"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200886"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjr.2016.07.005"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/dev.20419"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwp061"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1810291"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2004-0025"},{"key":"e_1_2_7_43_1","first-page":"5","article-title":"Ecolog\u00eda de la vizcacha (\"Lagostumus maximus maximus\" Blainv.) en el nordeste de la provincia de Entre R\u00edos","volume":"10","author":"Llanos A. C.","year":"1952","journal-title":"Revista de Investigaciones Agr\u00edcolas Extra Nueva Serie"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2016.00039"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0953-8194.2004.01167.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.01806"},{"key":"e_1_2_7_47_1","first-page":"55","article-title":"Melatonina testicular: Papel en la ciclicidad reproductiva","volume":"8","author":"Mu\u00f1oz E.","year":"1999","journal-title":"Revista de la Sociedad Argentina de Androlog\u00eda Afiliada a la International Society of Andrology"},{"key":"e_1_2_7_48_1","first-page":"31","article-title":"Changes in weight and hydroxyindole\u2010O\u2010methyltransferase activity of pineal gland of the plains viscacha (Lagostomus maximus maximus)","volume":"6","author":"Pelzer L. E.","year":"1999","journal-title":"Mastozoolgia Neotropical"},{"issue":"3","key":"e_1_2_7_49_1","first-page":"273","article-title":"Biological role of the pineal. Responses to the environmental photoperiod","volume":"17","author":"Piezzi R. S.","year":"1984","journal-title":"Archivos de Biologia y Medicina Experimental"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.2307\/3579833"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X1999000900010"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1009"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12280"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD18423"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0228-0"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030140"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.4074"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703779114"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-021048"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1159\/000122085"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1177\/074873049701200605"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0250365"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2008.07.013"},{"issue":"2","key":"e_1_2_7_64_1","first-page":"109","article-title":"Glucocorticoids, stress, and fertility","volume":"35","author":"Whirledge S.","year":"2010","journal-title":"Minerva Endocrinologica"},{"key":"e_1_2_7_65_1","volume-title":"Endotext [Internet]","author":"Willenberg H. S.","year":"2017"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.08.041"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(98)00067-7"},{"issue":"2","key":"e_1_2_7_68_1","first-page":"241","article-title":"Effects of melatonin on adrenal function in male rats","volume":"69","author":"Yamada K.","year":"1990","journal-title":"Research Communications in Chemical Pathology and Pharmacology"},{"issue":"4","key":"e_1_2_7_69_1","first-page":"629","article-title":"Stress\u2010induced changes in the aged\u2010rat adrenal cortex. Histological and histomorphometric study","volume":"77","author":"Zaki S. M.","year":"2018","journal-title":"Folia Morphologica"},{"issue":"1","key":"e_1_2_7_70_1","first-page":"107","article-title":"A Study of Adrenal Cortex Morphology","volume":"12","author":"Zwemer R. L.","year":"1936","journal-title":"The American Journal of Pathology"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.24457","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24457","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.24457","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24457","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T11:34:27Z","timestamp":1759923267000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.24457"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,18]]},"references-count":69,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1002\/ar.24457"],"URL":"https:\/\/doi.org\/10.1002\/ar.24457","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,18]]},"assertion":[{"value":"2019-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}