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These effects are also involved in the pathogenesis of asthma. However, the effect of isorhamnetin on bronchial epithelial cells in patients with asthma has not been examined. Cells of human bronchial epithelial cell line BEAS\u20102B were cultured with isorhamnetin and tumor necrosis factor (TNF)\u2010\u03b1. The effects of isorhamnetin on BEAS\u20102B cell viability were assessed using CCK8 assay. The EdU (5\u2010ethynyl\u20102\u2032\u2010deoxyuridine) cell proliferation assay was performed to assess cell proliferation. BEAS\u20102B cell migration was measured using Transwell and wound healing assays. Real\u2010time PCR and enzyme\u2010linked immunosorbent assay were conducted to measure the expression of pro\u2010inflammatory cytokines. Protein expression levels were determined by western blotting. Immunofluorescence was used to detect nuclear translocation of nuclear factor kappa B (NF\u2010\u03baB). We found that isorhamnetin at 20 and 40\u2009\u03bcM reduced the proliferation of BEAS\u20102B cells induced by TNF\u2010\u03b1. Isorhamnetin significantly decreased the expression of interleukin (IL)\u20101\u03b2, IL\u20106, IL\u20108, and C\u2010X\u2010C motif chemokine ligand 10 in BEAS\u20102B cells induced by TNF\u2010\u03b1. Additionally, 10 \u03bcM isorhamnetin effectively reduced cell migration induced by TNF\u2010\u03b1. Treatment with isorhamnetin inhibited the phosphorylation of mitogen\u2010activated protein kinase (MAPK) and NF\u2010\u03baB pathways induced by TNF\u2010\u03b1. In summary, isorhamnetin inhibited the inflammation, proliferation, and migration of BEAS\u20102B cells by regulating the MAPK and NF\u2010\u03baB signaling pathways and is a drug candidate for asthma.<\/jats:p>","DOI":"10.1002\/ar.24506","type":"journal-article","created":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T05:37:36Z","timestamp":1598852256000},"page":"901-913","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Isorhamnetin attenuates <scp>TNF<\/scp>\u2010\u03b1\u2010induced inflammation, proliferation, and migration in human bronchial epithelial cells via <scp>MAPK<\/scp> and <scp>NF\u2010\u03baB<\/scp> pathways"],"prefix":"10.1002","volume":"304","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2532-7600","authenticated-orcid":false,"given":"Xiaojie","family":"Ren","sequence":"first","affiliation":[{"name":"The First Clinical School, Guangzhou University of Chinese Medicine  Guangzhou Guangdong 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