{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:52:51Z","timestamp":1777632771204,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401688"],"award-info":[{"award-number":["81401688"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we present a new model for demyelination of the central nervous system (CNS). BALB\/c mice were infected with <jats:italic>Angiostrongylus cantonensis<\/jats:italic> and analyzed 7, 14, and 21\u2009days postinfection. Neurological scale evaluation, magnetic resonance imaging (MRI), histology, real\u2010time quantitative polymerase chain reaction, and western blotting were all performed on days 7, 14, and 21. The results showed that the neurological functions and weight of <jats:italic>A. cantonensis<\/jats:italic>\u2010infected mice decreased markedly after 21\u2009days of infection. MRI showed subdural effusion and white high signals in the corpus callosum in both T1WI and T2WI, while hematoxylin and eosin and luxol fast blue staining showed hemorrhage and demyelination in the corpus callosum. Transmission electron microscopy revealed that the ultrastructure of the myelin sheath in the corpus callosum was dispersed or disintegrated. The percentage of myelinated axons was significantly decreased, and the <jats:italic>g<\/jats:italic>\u2010ratio was lower than that in the normal group. Both protein and mRNA levels of myelin basic protein decreased markedly at 21\u2009days postinfection. Immunofluorescence revealed that the number of CC1 positive cells in the corpus callosum also decreased, which confirmed the damage of <jats:italic>A. cantonensis<\/jats:italic> to oligodendrocytes. Our experiments confirmed that <jats:italic>A. cantonensis<\/jats:italic> infection caused demyelination in the CNS of BALB\/c mice after 21\u2009days, and its clinical manifestations and pathological changes were similar to those of multiple sclerosis and other CNS demyelination models. Thus, mice infected with <jats:italic>A. cantonensis<\/jats:italic> could be used as a new model to study acute demyelination of the CNS.<\/jats:p>","DOI":"10.1002\/ar.24538","type":"journal-article","created":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T09:16:25Z","timestamp":1602926185000},"page":"1084-1093","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["BALB\/c mice infected with <i>Angiostrongylus cantonensis<\/i>: A new model for demyelination in the brain"],"prefix":"10.1002","volume":"304","author":[{"given":"Huihui","family":"Xiong","sequence":"first","affiliation":[{"name":"Histology and Embryology Department of Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongpu","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Medicine South China University of Technology  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongdao","family":"Wu","sequence":"additional","affiliation":[{"name":"Parasitology Department of Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou China"},{"name":"Key Laboratory of Tropical Disease Control (SYSU) Ministry of Education  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Medicine South China University of Technology  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fukang","family":"Xie","sequence":"additional","affiliation":[{"name":"Histology and Embryology Department of Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S003118201900101X"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458518805246"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.18-0808"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1440-6"},{"key":"e_1_2_9_6_1","first-page":"527","article-title":"Progressive multiple sclerosis: From pathogenic mechanisms to treatment","volume":"140","author":"Correale J.","year":"2017","journal-title":"Brain"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2406.180168"},{"key":"e_1_2_9_8_1","first-page":"569","article-title":"Eosinophilic meningitis caused by Angiostrongylus cantonensis\u2014A neglected disease with escalating importance","volume":"31","author":"Eamsobhana P.","year":"2014","journal-title":"Tropical Biomedicine"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-339"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2480"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.540"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2019.101663"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.03.019"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2009.05.006"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000608"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00904"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2013.07.010"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2565-y"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.schres.2014.04.041"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1179\/016164102101200276"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.24528"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2019.116245"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_843_18"},{"key":"e_1_2_9_24_1","first-page":"599","article-title":"The susceptibility of five stains mice to infections with Angiostrongylus cantonensis","volume":"11","author":"Wang J. 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