{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:38:50Z","timestamp":1762868330908,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T00:00:00Z","timestamp":1605225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302012\u20102017\u20106"],"award-info":[{"award-number":["302012\u20102017\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/24051\u20104"],"award-info":[{"award-number":["2017\/24051\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present study we investigated the involvement of free fatty acid (FFA) receptors in the anti\u2010inflammatory role of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in dystrophic muscles, by administering FFA blockers in the <jats:italic>mdx<\/jats:italic> mouse model of dystrophy. <jats:italic>Mdx<\/jats:italic> mice (3\u00a0months\u2010old) were treated with fish oil capsules (FDC Vitamins; 0.4\u00a0g EPA and 0.2\u00a0g DHA; gavage) alone or concomitant to FFA1 and FFA4 blockers (GW1100 and AH7614; i.p.). C57BL\/10 mice (3\u00a0months\u2010old) and untreated\u2010<jats:italic>mdx<\/jats:italic> mice received mineral oil and were used as controls. After 1\u00a0month of treatment, plasma markers of myonecrosis (total and cardiac creatine kinase; CK), the levels of FFA1 and FFA4 and of the markers of inflammation, nuclear transcription factor kappa B (NFkB), tumor necrosis factor alpha (TNF\u2010\u03b1) and interleukin 1\u03b2 (IL\u20101\u03b2) were analyzed in the diaphragm muscle and heart by western blot. Fish oil significantly reduced total CK, cardiac CK and the levels of NFkB (diaphragm), and of TNF\u2010\u03b1 and IL\u20101\u03b2 (diaphragm and heart) in <jats:italic>mdx<\/jats:italic>. In the dystrophic diaphragm, FFA1 was increased compared to normal. Blockers of FFA1 and FFA4 significantly inhibited the effects of fish oil treatment in both dystrophic muscles. The anti\u2010inflammatory effects of fish oil in dystrophic diaphragm muscle and heart were mediated through FFA1 and FFA4.<\/jats:p>","DOI":"10.1002\/ar.24563","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T11:07:23Z","timestamp":1604315243000},"page":"1305-1312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fish oil attenuated dystrophic muscle markers of inflammation via <scp>FFA1<\/scp> and <scp>FFA4<\/scp> in the <i>mdx<\/i> mouse model of <scp>DMD<\/scp>"],"prefix":"10.1002","volume":"304","author":[{"given":"Marcos","family":"Maciel Junior","sequence":"first","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology, University of Campinas (Unicamp)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samara","family":"Cama\u00e7ari de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology, University of Campinas (Unicamp)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Andrea","family":"Saenz Suarez","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology, University of Campinas (Unicamp)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto","family":"Santo Neto","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology, University of Campinas (Unicamp)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4894-4401","authenticated-orcid":false,"given":"Maria Julia","family":"Marques","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology, University of Campinas (Unicamp)  Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706770"},{"key":"e_1_2_7_3_1","first-page":"1","article-title":"N\u2010acetylcysteine decreases fibrosis and increases force\u2010generating capacity of mdx diaphragm","volume":"8","author":"Burns D. P.","year":"2019","journal-title":"Antioxidants (Basel)"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2012.04374.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2013.09.007"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-015-0864-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335820"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2013.11.021"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2017.12.006"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnesp.2017.03.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2012.11.013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2016.01.015"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1168"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000008168"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.112.300943"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01478"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.657379"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)03064-4"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.15.75"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-235333"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.114.218974"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2019.00144"},{"key":"e_1_2_7_23_1","first-page":"1","article-title":"Omega\u20103 and omega\u20106 PUFAs induce the same GPR120\u2010mediated signalling events, but with different kinetics and intensity in Caco\u20102 cells","volume":"13","author":"Mobraten K.","year":"2013","journal-title":"Lipids in Health and Disease"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.01.021"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e3283479230"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.07.041"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2010.09.013"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-017-0043-3"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2018.10.017"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2017.09.011"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11102279"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.00755"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12476"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/352536a0"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23468"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-019-09758-8"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2006.04394.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2014.12.011"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24563","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.24563","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24563","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:43:25Z","timestamp":1759949005000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.24563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,13]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1002\/ar.24563"],"URL":"https:\/\/doi.org\/10.1002\/ar.24563","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2020,11,13]]},"assertion":[{"value":"2020-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}