{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T22:56:54Z","timestamp":1769727414324,"version":"3.49.0"},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spleen\u2010stomach dampness\u2010heat syndrome (SSDHS) is the common Traditional Chinese Medicine (TCM) syndrome observed in both chronic hepatitis B (CHB) and chronic gastritis (CG). The specialized TCM prescription for CHB and CG patients with SSDHS is same, but there is limited information about the biological characteristics of this TCM syndrome. This study aimed to identify the serum miRNAs profile for the SSDHS in two different diseases in order to evaluate the miRNA\u2010mediated biological characteristics of this TCM syndrome. We performed comparative microarray analysis of serum miRNA expression profiles in 10 CHB patients with SSDHS (SSDHS\u2010CHB), 10 CG patients with SSDHS (SSDHS\u2010CG), and 10 healthy controls (HC). The selected miRNAs were further validated by qRT\u2010PCR in 13 SSDHS\u2010CHB patients, 13 SSDHS\u2010CG patients, and 13 HC. Moreover, bioinformatics analysis (GO and KEGG pathway enrichment analyses) was applied to identify the involved target genes and pathways for these selected miRNAs. Nine significantly differentially expressed (SDE)\u2010miRNAs in the SSDHS\u2010CHB group and 24 SDE\u2010miRNAs in the SSDHS\u2010CG group were identified, compared with the HC group (fold change &gt;2.0 and <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Among these, upregulated hsa\u2010miR\u2010483\u20103p and downregulated hsa\u2010miR\u2010223\u20103p were identified as the common SDE\u2010miRNAs for both SSDHS\u2010CHB and SSDHS\u2010CG groups. Bioinformatics analysis of the common SDE\u2010miRNA's target genes showed their involvement in the regulation of inflammation, immune response, and tumorigenesis. SSDHS\u2010specific hsa\u2010miR\u2010483\u20103p and hsa\u2010miR\u2010223\u20103p identified in this study indicated a relevance to the underlying biological basis of SSDHS, and may provide scientific basis for the application of same TCM prescription in CHB and CG.<\/jats:p>","DOI":"10.1002\/ar.24690","type":"journal-article","created":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T07:10:24Z","timestamp":1626765024000},"page":"2620-2631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative serum <scp>microRNA<\/scp> array analysis of the <scp>spleen\u2010stomach dampness\u2010heat<\/scp> syndrome in different diseases: Chronic hepatitis B and chronic gastritis"],"prefix":"10.1002","volume":"304","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1459-8803","authenticated-orcid":false,"given":"Baixue","family":"Li","sequence":"first","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Su","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ne","family":"Xiang","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiyu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingjun","family":"Wan","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiyang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cen","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan\u2010Sheng","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,20]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Chen G. 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