{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:58:05Z","timestamp":1759971485121,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T00:00:00Z","timestamp":1626393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009110","name":"Natural Science Foundation of Xinjiang Province","doi-asserted-by":"publisher","award":["2018D01C158"],"award-info":[{"award-number":["2018D01C158"]}],"id":[{"id":"10.13039\/100009110","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endemic fluorosis is a chronic systemic disease that seriously endangers human health. In high fluoride areas, people consume excessive fluoride for a long time through drinking water or food, which leads to chronic cumulative fluorosis in the body. Fluorosis can cause changes in the expression of some miRNA in cells, and the miRNA can participate in fluoride\u2010induced osteoblast activation through various signal pathways. To observe the differential expression of apoptosis\u2010related microRNA (miRNA) in mouse osteoblasts under the action of excessive fluoride. Primary cultured mouse osteoblasts, identified by osteocalcin (OC) and alkaline phosphatase (ALP) staining, were treated with 20\u2009mg\/L sodium fluoride and 40\u2009mg\/L sodium fluoride for 12\/24\u2009hr, respectively, to establish the fluoride staining model for comparing and analyzing the sequence of miRNA among groups by bioinformatics methods; four miRNA chains were verified by fluorescence quantitative PCR. After treatment with 20\u2009mg\/L sodium fluoride for 12\u2009hr and 24\u2009hr, 128 miRNA expressions were up\u2010regulated while 36 miRNA expressions were down\u2010regulated. In Group 40\u2009mg\/L, 130 miRNA expressions were up\u2010regulated while 29 miRNA expressions were down\u2010regulated after 12\u2009hr and 24\u2009hr; 72 miRNA were up\u2010regulated and 2 miRNA were down\u2010regulated at the two time points. 10 up\u2010regulated miRNA and 2 down\u2010regulated miRNA with higher scores in Bioinformatics software were analyzed the target genes. Fluorescence quantitative PCR verified that the expressions of four miRNA were up\u2010regulated. Target gene analysis of the 10 selected mouse osteoblastic apoptosis\u2010related miRNA reveals their involvement of the functions of inhibiting or promoting apoptosis, which has certain theoretical significance for early identification of skeletal fluorosis. The involved signaling pathways include the Wnt signaling pathway, ubiquitin\u2010regulated proteolysis, Toll signaling pathway, TNF signaling pathway, pluripotent stem cell signaling pathway, MAPK signaling pathway, phosphatidylinositide metabolism, FoxO signaling pathway, ErbB signaling pathway, autophagy, and so forth.<\/jats:p>","DOI":"10.1002\/ar.24709","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T05:06:34Z","timestamp":1625720794000},"page":"359-372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preliminary screening of fluorine\u2010stained osteoblastic apoptosis\u2010related <scp>microRNA<\/scp>"],"prefix":"10.1002","volume":"305","author":[{"given":"Qiang","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Zhouyang","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weibin","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shutao","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1559-3243","authenticated-orcid":false,"given":"Yalou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Histology &amp; Embryology, School of Basic Medical Sciences Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luan","family":"Haopeng","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery First Affiliated Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bai","family":"Bingxin","sequence":"additional","affiliation":[{"name":"The Fifth Teaching Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Mengting","sequence":"additional","affiliation":[{"name":"The Fifth Teaching Hospital of Xinjiang Medical University  Xinjiang Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00653"},{"issue":"6","key":"e_1_2_6_3_1","first-page":"638","article-title":"Correlation study between serum bone turnover markers and gender and blood glucose status in patients with early stage osteoporotic thoracolumbar and hip","volume":"19","author":"Cao D. H.","year":"2020","journal-title":"Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-016-0871-4"},{"key":"e_1_2_6_5_1","first-page":"1149","article-title":"Differential expression of microRNAs related to apoptosis in human osteoblasts induced by sodium fluoride","volume":"8","author":"Deng Q.","year":"2018","journal-title":"Chinese Journal of Tissue Engineering Research"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2015.3054"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-019-01684-9"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.01.095"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.02.015"},{"issue":"5","key":"e_1_2_6_10_1","first-page":"196","article-title":"Knowledge of primary care professionals about fluoride topics","volume":"67","author":"Lima V. V.","year":"2018","journal-title":"Minerva Stomatologica"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-339"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17328-8"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-018-0291-8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0179-x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-016-0833-x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12575-016-0037-y"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24709","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.24709","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:26:13Z","timestamp":1759933573000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.24709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,16]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/ar.24709"],"URL":"https:\/\/doi.org\/10.1002\/ar.24709","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2021,7,16]]},"assertion":[{"value":"2021-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}