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The c\u2010Jun\u2010NH(2)\u2010terminal kinase (JNK) signaling pathway is considered to be involved in the antidepressant mechanism of EA. However, the antidepressant effect of EA via modulating the expression of c\u2010Fos\/activator protein\u20101 (AP\u20101) under the condition of JNK inhibition remains unexplored. In this study, we investigated the antidepressant effect and possible mechanism of EA in regulating the expression of c\u2010Fos\/AP\u20101 under the condition of JNK inhibition by SP600125 in rats exposed to chronic unpredictable mild stress (CUMS). The depression\u2010like behaviors were evaluated by the body weight, sucrose preference test (SPT), and open field test (OFT). The expression levels of c\u2010Jun in the hypothalamus, c\u2010Fos in the pituitary gland, and c\u2010Fos and AP\u20101 in the serum of CUMS induced rat model of depression were detected by ELISA. The results indicated that treatment with EA and fluoxetine can reverse the CUMS\u2010induced depression\u2010like behaviors in rats and can up\u2010regulate the expression levels of c\u2010Jun in the hypothalamus, c\u2010Fos in the pituitary gland, and c\u2010Fos and AP\u20101 in the serum. Of note, the data demonstrated that SP600125, the inhibitor of JNK signaling pathway, can exert synergistic effect with EA in regulating CUMS\u2010induced abnormal activation of the JNK signaling pathway. The antidepressant effect of EA might be mediated by modulating the expression of c\u2010Fos\/AP\u20101.<\/jats:p>","DOI":"10.1002\/ar.24740","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T06:55:13Z","timestamp":1629874513000},"page":"2480-2493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antidepressant effect of electroacupuncture on modulating the expression of <scp>c\u2010Fos<\/scp>\/<scp>AP<\/scp>\u20101 through the <scp>JNK<\/scp> signaling pathway"],"prefix":"10.1002","volume":"304","author":[{"given":"Xiang","family":"Li","sequence":"first","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Beijing China"},{"name":"Research Center of Mental and Neurological Disorders School of Acupuncture\u2010Moxibustion and Tuina, Beijing University of Chinese Medicine  Beijing 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Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Shan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Hong","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u2010Ya","family":"Bao","sequence":"additional","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Beijing China"},{"name":"Research Center of Mental and Neurological Disorders School of Acupuncture\u2010Moxibustion and Tuina, Beijing University of Chinese Medicine  Beijing 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