{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T18:39:35Z","timestamp":1792348775107,"version":"4.4.0"},"reference-count":55,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A030312009"],"award-info":[{"award-number":["2017A030312009"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Atractylodeslancea<\/jats:italic> Rhizoma (Rhizoma atractylodis [RA]) has long been recommended for the treatment of arthritis in traditional Chinese medicine, but its mechanism of action is still unclear. RA contains a large amount of <jats:italic>Atractylodes lancea<\/jats:italic> volatile oils (Atr). In this study, we investigated whether Atr can promote mesenchymal stem cells (MSCs) chondrogenic differentiation. The Atr were extracted from RA by steam distillation method, and the effect of Atr on MSCs was detected by the CCK8 assay. The optimal concentration of Atr for MSCs cultivation was 3\u00a0\u03bcg\/ml. The differentially expressed miR\u2010181a\u20105p was screened by miRNA microarray assay, and its mimics and inhibitors were transfected into MSCs. It was found that the inhibitor of miR\u2010181a\u20105p could upregulate cartilage\u2010specific genes such as <jats:italic>SOX9<\/jats:italic>, <jats:italic>COL2A1<\/jats:italic>, and <jats:italic>ACAN<\/jats:italic>. Meanwhile, we also found that the expression of gene editing enzyme ADAR2 was significantly increased in the chondrogenic differentiation of MSCs induced by Atr, and the bases of precursor sequence of miR\u2010181a\u20105p were changed from A to G. After ADAR2 deletion, the expression of cartilage\u2010specific genes was significantly down\u2010regulated and the precursor sequence bases of miR\u2010181a\u20105p were not changed. Bioinformatics analysis revealed that the predicted target gene of miR\u2010181a\u20105p was yingyang1 (YY1), and the targeting relationship was verified by dual\u2010luciferase reporter assay. After deleting YY1, the expression of cartilage\u2010specific genes was significantly down\u2010regulated. In conclusion, our study demonstrated that Atr can promote chondrogenic differentiation of MSC through regulation of the ADAR2\u2010miR\u2010181a\u20105p signaling pathway. This may provide a new insight into the possible mechanism of traditional Chinese medicine (Atr) in treating inflammatory joint diseases.<\/jats:p>","DOI":"10.1002\/ar.24930","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T10:28:53Z","timestamp":1650536933000},"page":"3006-3020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Atractylodes lancea<\/i> volatile oils target ADAR2\u2010miR\u2010181a\u20105p signaling to mesenchymal stem cell chondrogenic differentiation"],"prefix":"10.1002","volume":"306","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6602-6524","authenticated-orcid":false,"given":"Shanyu","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Traditional Chinese Medicine Innovation Research Center, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine Guangzhou University of Chinese Medicine  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Si","sequence":"additional","affiliation":[{"name":"Shenzhen BaoAn Traditional Chinese Medicine Hospital Guangzhou University of Chinese Medicine  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5242-4150","authenticated-orcid":false,"given":"Ziwei","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulu","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrong","family":"Li","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Innovation Research Center, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine Guangzhou University of Chinese Medicine  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1199-291X","authenticated-orcid":false,"given":"Zifeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Innovation Research Center, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine Guangzhou University of Chinese Medicine  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Zhu","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Innovation Research Center, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine Guangzhou University of Chinese Medicine  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongfeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Basic Medical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.116319"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2013.109"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603518764262"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0712(21)00667-3"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b05841"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.24"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18040799"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc20167"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.12670"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.02.030"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2012.197"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevOncog.2017021311"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt247"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297919080054"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.10.015"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.11.099"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/35017558"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.9b00025"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.55664"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pat.4869"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2016.07.011"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.11.038"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.009409"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-00265"},{"key":"e_1_2_12_27_1","first-page":"1279","article-title":"miR3395p negatively regulates loureirin Ainduced hair follicle stem cell differentiation by targeting DLX5","volume":"18","author":"Li X.","year":"2018","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201600574"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.26981"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24480"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7080092"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2019.00009"},{"key":"e_1_2_12_33_1","first-page":"S44","article-title":"MiR\u2010140 is expressed in differentiated human articular chondrocytes and modulates IL\u20101 responses","volume":"17","author":"Miyaki S","year":"2009","journal-title":"Arthritis & Rheumatology"},{"issue":"1","key":"e_1_2_12_34_1","first-page":"111","article-title":"microRNA\u2010181a\u20105p antisense oligonucleotides attenuate osteoarthritis in facet and knee joints","volume":"78","author":"Nakamura A.","year":"2019","journal-title":"Basic and Translational Research"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.86820"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.8.4.15390"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060208-105251"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.21.9804"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13652"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1633291100"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.134"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2014.0661"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14621-5"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0575-z"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22957"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2014.1743"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2016-0078"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI123396"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.04.054"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1041"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.01.024"},{"key":"e_1_2_12_52_1","first-page":"4689","article-title":"MicroRNA\u201030a regulates chondrogenic differentiation of human bone marrow\u2010derived mesenchymal stem cells through targeting Sox9","volume":"18","author":"Zhang H.","year":"2019","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_12_53_1","first-page":"6275","article-title":"Mesenchymal stem cell related therapies for cartilage lesions and osteoarthritis","volume":"11","author":"Zhang R.","year":"2019","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113415"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2015.01.060"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.152922"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24930","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.24930","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.24930","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:15:31Z","timestamp":1759943731000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.24930"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,21]]},"references-count":55,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/ar.24930"],"URL":"https:\/\/doi.org\/10.1002\/ar.24930","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,21]]},"assertion":[{"value":"2021-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}