{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:31:37Z","timestamp":1786977097095,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T00:00:00Z","timestamp":1651449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neurons, expressing neuronal nitric oxide synthase (nNOS) in the enteric ganglia are inhibitory motor neurons or interneurons. The aim of the study was to identify the percentage, cross\u2010sectional area of nNOS\u2010immunoreactive (IR) neurons and their colocalization with choline acetyltransferase (ChAT), vasoactive intestinal polypeptide (VIP), and neuropeptide Y in the intramural ganglia of the myenteric (MP) and submucous plexus (SP) of the small intestine (SI) and large intestine (LI) of rats of different age groups using immunohistochemical methods. In the intramural ganglia of the MP, the largest percentage of nNOS\u2010IR neurons was detected in newborn rats in the LI (81\u2009\u00b1\u20090.9%) and SI (48\u2009\u00b1\u20094.1%). Subsequently, it decreased in ontogenesis up to 60\u2009days of life (26\u2009\u00b1\u20090.9% LI, 29\u2009\u00b1\u20093.2% SI), and did not change until senescence. In the SP, abundant nNOS\u2010IR neurons were also detected in newborns (82\u2009\u00b1\u20097.0% SI, 85\u2009\u00b1\u20093.2% LI), while their percentage decreased significantly in the next 20\u2009days. Furthermore, a very small number of nNOS\u2010IR neurons was detected in 30\u2010day\u2010 and 2\u2010month\u2010old animals, but they again appeared in large numbers in aged rats. In the MP, the highest percentage of nNOS+\/ChAT+ neurons was in 1\u2010day\u2010old, 10\u2010day\u2010old, and 2\u2010year\u2010old rats. In the SP, the largest number of nNOS\u2010IR neurons colocalized ChAT regardless of age. In the MP of all rats, many nNOS\u2010IR neurons colocalized VIP, and the maximal percentage of nNOS+\/VIP+ neurons was found in 2\u2010year\u2010old rats, minimal\u2014in newborns. In conclusion, nNOS expression in neurons of the gut is decreased in early postnatal ontogenesis and subsequently increased in aged rats.<\/jats:p>","DOI":"10.1002\/ar.24947","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T14:29:26Z","timestamp":1651501766000},"page":"2276-2291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase"],"prefix":"10.1002","volume":"306","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3333-5865","authenticated-orcid":false,"given":"Antonina F.","family":"Budnik","sequence":"first","affiliation":[{"name":"Department of Normal and Pathological Anatomy Kabardino\u2010Balkarian State University  Nalchik Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6230-5024","authenticated-orcid":false,"given":"Petr M.","family":"Masliukov","sequence":"additional","affiliation":[{"name":"Department of Normal Physiology Yaroslavl State Medical University  Yaroslavl Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.647555"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.219717"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23423"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-020-04689-x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2019.101982"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.5603\/FM.2015.0028"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59259-465-8_7"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2006.10.005"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apha.2017.09.003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00462.2010"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1979.tb00111.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2008.05.005"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-27592-5_13"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.265686"},{"key":"e_1_2_10_17_1","volume-title":"The enteric nervous system","author":"Furness J. 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