{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:06:21Z","timestamp":1759971981629,"version":"build-2065373602"},"reference-count":69,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T00:00:00Z","timestamp":1652745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["355316","RGPIN\u20102018\u201005100"],"award-info":[{"award-number":["355316","RGPIN\u20102018\u201005100"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Shigella flexneri<\/jats:italic> (<jats:italic>S. flexneri<\/jats:italic>), the causative agent of bacillary dysentery, uses an effector\u2010mediated strategy to hijack host cells and cause disease. To propagate and spread within human tissues, <jats:italic>S. flexneri<\/jats:italic> bacteria commandeer the host actin cytoskeleton to generate slender actin\u2010rich comet tails to move intracellularly, and later, plasma membrane actin\u2010based protrusions to move directly between adjacent host cells. To facilitate intercellular bacterial spreading, large micron\u2010sized endocytic\u2010like membrane invaginations form at the periphery of neighboring host cells that come into contact with <jats:italic>S. flexneri\u2010<\/jats:italic>containing membrane protrusions. While <jats:italic>S. flexneri<\/jats:italic> comet tails and membrane protrusions consist primarily of host actin cytoskeletal proteins, <jats:italic>S. flexneri<\/jats:italic> membrane invaginations remain poorly understood with only clathrin and the clathrin adapter epsin\u20101 localized to the structures. Tangentially, we recently reported that <jats:italic>Listeria monocytogenes,<\/jats:italic> another actin\u2010hijacking pathogen, exploits an assortment of caveolar and actin\u2010bundling proteins at their micron\u2010sized membrane invaginations formed during their cell\u2010to\u2010cell movement. Thus, to further characterize the <jats:italic>S. flexneri<\/jats:italic> disease process, we set out to catalog the distribution of a variety of actin\u2010associated and caveolar proteins during <jats:italic>S. flexneri<\/jats:italic> actin\u2010based motility and cell\u2010to\u2010cell spreading. Here we show that actin\u2010associated proteins found at <jats:italic>L. monocytogenes<\/jats:italic> comet tails and membrane protrusions mimic those present at <jats:italic>S. flexneri<\/jats:italic> comet tails with the exception of \u03b1\u2010actinins 1 and 4, which were shed from <jats:italic>S. flexneri<\/jats:italic> membrane protrusions. We also demonstrate that all known host endocytic components found at <jats:italic>L. monocytogenes<\/jats:italic> membrane invaginations are also present at those formed during <jats:italic>S. flexneri<\/jats:italic> infections.<\/jats:p>","DOI":"10.1002\/ar.24955","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T02:15:22Z","timestamp":1652840122000},"page":"1088-1110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Localization of host endocytic and actin\u2010associated proteins during <i>Shigella flexneri<\/i> intracellular motility and intercellular spreading"],"prefix":"10.1002","volume":"306","author":[{"given":"Aaron Singh","family":"Dhanda","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease Simon Fraser University  Burnaby British Columbia 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