{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T08:58:30Z","timestamp":1769763510232,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T00:00:00Z","timestamp":1655856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo Municipality","doi-asserted-by":"publisher","award":["202003N4186"],"award-info":[{"award-number":["202003N4186"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo Municipality","doi-asserted-by":"publisher","award":["202003N4215"],"award-info":[{"award-number":["202003N4215"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY18H270003"],"award-info":[{"award-number":["LY18H270003"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Huangqi decoction, also known as Huangqi Liuyi decoction, was first recorded in the prescriptions of the Bureau of Taiping People's Welfare Pharmacy. It comprises astragalus and licorice, which is a commonly used prescription in traditional Chinese medicine for the clinical treatment of chronic liver disease, especially liver cirrhosis. Total astragalus saponins (AST) is the main component of astragalus, and glycyrrhizic acid (GA) is the main component of licorice. In this study, normal macrophage exosomes were extracted, and the exosomes incubated with lipopolysaccharides (LPS) and those incubated with LPS\u2009+\u2009AST\u2009+\u2009GA were co\u2010cultured with JS1 cells (hepatic stellate cell line). The survival rate and the activation of key signaling pathways of JS1 cells in each group were detected and compared. We found that the co\u2010culture of LPS\u2010induced macrophage exosomes with JS1 cells could significantly increase the expression levels of Collagen\u20101 (Col\u20101) and Alpha smooth muscle actin (\u03b1\u2010SMA)in JS1 cells. However, a significant reversal effect was observed after pretreatment with AST combined with GA. Further evaluation found that the expression levels of phospho (p)\u2010Smad2 and p\u2010Smad3 in the JS1 cells were significantly increased after macrophages were induced with LPS, whereas pretreatment with AST\u2009+\u2009GA could significantly decrease the expression levels of p\u2010Smad2 and p\u2010Smad3. Preliminary results of this study indicated that LPS\u2010induced macrophage exosomes can promote the activation of hepatic stellate cells, and the pretreatment of AST combined with GA can exert a significant intervention effect. In this study, the new mechanism of anti\u2010hepatic fibrosis effect of traditional Chinese medicine components of Huangqi Decoction was analyzed from the perspective of exosomes.<\/jats:p>","DOI":"10.1002\/ar.25009","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T08:31:37Z","timestamp":1655886697000},"page":"3097-3105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>LPS<\/scp>\u2010induced macrophage exosomes promote the activation of hepatic stellate cells and the intervention study of total astragalus saponins combined with glycyrrhizic acid"],"prefix":"10.1002","volume":"306","author":[{"given":"Kaili","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Medical School Ningbo University  Ningbo China"},{"name":"School of Medicine Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Medical School Ningbo University  Ningbo China"},{"name":"School of Medicine Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Medical School Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongxue","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Medical School Ningbo University  Ningbo China"},{"name":"Institute of Digestive Disease of Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0693-4747","authenticated-orcid":false,"given":"Yuping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Hospital of Medical School Ningbo University  Ningbo China"},{"name":"Institute of Digestive Disease of Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17030376"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2014.04.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902307RRR"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.01.042"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.11.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02225-16"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs1040027"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2016.1251309"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri978"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.31658"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-020-00836-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-00381-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16123"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-00372-7"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29803"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03389-2"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3020-0"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.064"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X1750063X"},{"issue":"15","key":"e_1_2_8_22_1","first-page":"74","article-title":"Nicousamide attenuates renal dysfunction and glomerular injury in remnant kidneys by inhibiting TGF\u2010\u03b21 internalisation and renin activity","volume":"845","author":"Sen Z.","year":"2018","journal-title":"European Journal of Pharmacology"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28644"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X16500324"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-0383-9"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.41580"},{"key":"e_1_2_8_27_1","first-page":"981","article-title":"Advances in the regulation of functions of hepatic stellate cells by exosomes","volume":"28","author":"Ye Q. 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