{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T16:35:31Z","timestamp":1778430931723,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T00:00:00Z","timestamp":1655510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The enteric nervous system, a major subdivision of the autonomic nervous system, is known for its neurochemical heterogeneity and complexity. The myenteric plexus, one of its two principal components, primarily controls peristalsis and its dysfunction may lead to a number of gastrointestinal motility disorders. The myenteric neurons have been described to use a wide variety of neurotransmitters although no evidence has been reported for the existence of adrenergic neurons in the hindgut. This study aims at elucidating the chemical coding of neurons in the myenteric plexus of the rat colon and anorectal region with particular emphasis on cholinergic and the so\u2010called nonadrenergic, noncholinergic (NANC) transmitter systems. The immunostaining for choline acetyltransferase revealed an intense staining of the myenteric ganglia with clear delineation of their neuronal cell bodies and without local distributional differences in the colonic region. The myenteric ATPergic structures were mostly limited to fiber bundles surrounding unstained myenteric neurons and penetrating the two muscle layers. We also observed an abundance of intensely stained varicose substance P\u2010immunopositive fibers, ensheathing the immunonegative myenteric neuronal cell bodies in a basket\u2010like manner. Applying NADPH\u2010diaphorase histochemistry and nitric oxide synthase immunohistochemistry, we were able to demonstrate numerous nitrergic somata of myenteric neurons with Dogiel Type I morphology. Apart from the observed nitrergic distributional patterns, no distinct variations were found in the staining intensity or distribution of myenteric structures in the colon and anorectal area. Our results suggest that myenteric neurons in the distal intestinal portion utilize a broad spectrum of enteric transmitters, including classical and NANC transmitters.<\/jats:p>","DOI":"10.1002\/ar.25019","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T13:24:41Z","timestamp":1655558681000},"page":"2292-2301","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Neurochemical profile of the myenteric plexus in the rat colorectal region"],"prefix":"10.1002","volume":"306","author":[{"given":"Todor V.","family":"Kirov","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology Medical University of Sofia  Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrinka Y.","family":"Atanasova","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine Trakia University  Stara Zagora Bulgaria"},{"name":"Institute of Neurobiology Bulgarian Academy of Sciences  Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3420-4346","authenticated-orcid":false,"given":"Nikolai E.","family":"Lazarov","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology Medical University of Sofia  Sofia Bulgaria"},{"name":"Institute of Neurobiology Bulgarian Academy of Sciences  Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00305792"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(97)00012-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20082003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050267"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90050-X"},{"issue":"3","key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0031-6997(25)06930-3","article-title":"Purinergic nerves","volume":"24","author":"Burnstock G.","year":"1972","journal-title":"Pharmacological Reviews"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1970.tb10646.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i28.5068"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(00)00127-2"},{"key":"e_1_2_7_11_1","first-page":"29","volume-title":"The enteric nervous system","author":"Furness J. 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