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Quantitatively tracking spatiotemporal patterns of cartilage and bone degeneration is critical for assessment of more appropriately targeted OA therapies. In this study, we use contrast\u2010enhanced micro\u2010computed tomography (\u03bcCT) to establish a timeline of subchondral plate (SCP) and cartilage changes in the murine femur after destabilization of the medial meniscus (DMM). We performed DMM or sham surgery in 10\u201312\u2010week\u2010old male C57Bl\/6J mice. Femora were imaged using \u03bcCT after 0, 2, 4, or 8\u2009weeks. Cartilage\u2010optimized scans were performed after immersion in contrast agent CA4+. Bone mineral density distribution (BMDD), cartilage attenuation, SCP, and cartilage thickness and volume were measured, including lateral and medial femoral condyle and patellar groove compartments. As early as 2\u2009weeks post\u2010DMM, cartilage thickness significantly increased and cartilage attenuation, SCP volume, and BMDD mean significantly decreased. Trends in cartilage and SCP metrics within each joint compartment reflected those seen in global measurements, and both BMDD and SCP thickness were consistently greater in the lateral and medial condyles than the patellar groove. Sham surgery also resulted in significant changes to SCP and cartilage metrics, highlighting a potential limitation of using surgical models to study tissue morphology or composition changes during OA progression. Contrast\u2010enhanced \u03bcCT analysis is an effective tool to monitor changes in morphology and composition of cartilage, and when combined with bone\u2010optimized \u03bcCT, can be used to assess the progression of degenerative changes after joint injury.<\/jats:p>","DOI":"10.1002\/ar.25027","type":"journal-article","created":{"date-parts":[[2022,6,25]],"date-time":"2022-06-25T05:54:11Z","timestamp":1656136451000},"page":"92-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Contrast\u2010enhanced micro\u2010computed tomography of compartment and time\u2010dependent changes in femoral cartilage and subchondral plate in a murine model of osteoarthritis"],"prefix":"10.1002","volume":"306","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1508-1045","authenticated-orcid":false,"given":"Deva D.","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Rush University Medical Center  Chicago Illinois USA"},{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"},{"name":"Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute  Troy New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2847-2766","authenticated-orcid":false,"given":"Maleeha","family":"Mashiatulla","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3257-9315","authenticated-orcid":false,"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3760-234X","authenticated-orcid":false,"given":"Ryan D.","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2968-1746","authenticated-orcid":false,"given":"Meghana","family":"Pendyala","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute  Troy New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9323-3262","authenticated-orcid":false,"given":"Amit","family":"Patwa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Department of Chemistry Boston University  Boston Massachusetts USA"},{"name":"Department of Chemistry Boston University  Boston Massachusetts USA"},{"name":"Division of Chemistry Navrachana University  Vadodara Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5453-3668","authenticated-orcid":false,"given":"Mark W.","family":"Grinstaff","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Department of Chemistry Boston University  Boston Massachusetts USA"},{"name":"Department of Chemistry Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5205-0892","authenticated-orcid":false,"given":"Anna","family":"Plaas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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