{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:30:56Z","timestamp":1786977056935,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004387","name":"Ningbo University","doi-asserted-by":"publisher","award":["201804"],"award-info":[{"award-number":["201804"]}],"id":[{"id":"10.13039\/501100004387","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The detailed mechanism of inflammation in postoperative cognitive dysfunction (POCD) is unclear. This study aimed to determine whether electroacupuncture (EA) ameliorates POCD by modulating gut microbial dysbiosis. Compared to the control group, mice in the EA group were treated at the acupoints Zusanli (ST36), Quchi (L111), Baihui (GV20), and Dazhui (GV14) 1 week before appendectomy. Novel object recognition and the Morris water maze tests were used to assess learning and spatial reference memory deficits, whereas hippocampus samples and stool samples were collected for central inflammatory tests and 16S\u2010rRNA sequencing of intestinal flora, respectively. In amyloid precursor protein\/presenilin 1 (APP\/PS1) mice, EA enhanced spatial memory and learning deficits. The fecal microbial community was altered in APP\/PS1 mice in the absence of EA following surgery. Among them,\n                    <jats:italic>Coprococcus<\/jats:italic>\n                    and\n                    <jats:italic>Bacteroidetes<\/jats:italic>\n                    were more abundant in the EA groups than in the control groups; however,\n                    <jats:italic>Actinobacteriota<\/jats:italic>\n                    ,\n                    <jats:italic>Helicobacteraceae<\/jats:italic>\n                    , and\n                    <jats:italic>Escherichia<\/jats:italic>\n                    \/\n                    <jats:italic>shigella<\/jats:italic>\n                    constitute the minor bacterial colonization in the EA groups. Furthermore, we found a significant negative correlation between\n                    <jats:italic>Firmicutes<\/jats:italic>\n                    and escape latency (Pearson correlation coefficient \u2212 0.551,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01) and positive correlation between\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    and escape latency (Pearson correlation coefficient 0.462,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Electron microscopy revealed signs of blood\u2013brain barrier (BBB) impairments and immunofluorescence images showed glial cells activated in the hippocampus of APP\/PS mice without EA, and serum diamine oxidase levels were increased in these mice; whereas EA treatment significantly relieved the above pathological changes. Our findings implied that EA decreases hippocampal inflammation of\u00a0APP\/PS1 by upregulating benificial\u00a0 gut microbiota, reducing BBB and intestinal barrier dysfunction, thus alleviates postoperative cognitive dysfunction. This may provide a novel target in POCD management.\n                  <\/jats:p>","DOI":"10.1002\/ar.25065","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T08:36:39Z","timestamp":1662971799000},"page":"3131-3143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Electroacupuncture modulates gut microbiota in mice: A potential target in postoperative cognitive dysfunction"],"prefix":"10.1002","volume":"306","author":[{"given":"Binbin","family":"Zhu","sequence":"first","affiliation":[{"name":"The Department of Radiology and Anesthesiology The Affiliated Hospital of Medical School of Ningbo University  Ningbo Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Zhou","sequence":"additional","affiliation":[{"name":"The Department of Radiology and Anesthesiology The Affiliated Hospital of Medical School of Ningbo University  Ningbo Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijian","family":"Zhou","sequence":"additional","affiliation":[{"name":"The Department of Radiology and Anesthesiology The Affiliated Hospital of Medical School of Ningbo University  Ningbo Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunqu","family":"Chen","sequence":"additional","affiliation":[{"name":"The Department of Radiology and Anesthesiology The Affiliated Hospital of Medical School of Ningbo University  Ningbo Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8008-8740","authenticated-orcid":false,"given":"Jianhua","family":"Wang","sequence":"additional","affiliation":[{"name":"The Department of Radiology and Anesthesiology The Affiliated Hospital of Medical School of Ningbo University  Ningbo Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Xu","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine Ningbo University  Ningbo Jiangbei District China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine Ningbo University  Ningbo Jiangbei District China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.10.021"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172914"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.02.009"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2014.01.002"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2019.06.017"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2018.12.005"},{"key":"e_1_2_11_9_1","first-page":"110","article-title":"Cognitive reserve and the risk of postoperative cognitive dysfunction","volume":"114","author":"Feinkohl I.","year":"2017","journal-title":"Deutsches Arzteblatt International"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/393569"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2020.0106"},{"key":"e_1_2_11_12_1","first-page":"341","article-title":"Preventive electroacupuncture ameliorates D\u2010galactose\u2010induced Alzheimer's disease\u2010like inflammation and memory deficits, probably via modulating the microbiota\u2010gut\u2010brain axis","volume":"24","author":"He C.","year":"2021","journal-title":"Iranian Journal of Basic Medical Sciences"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2242074"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2021.806700"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2019.05.006"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/9796419"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2020.1814107"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-37"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnsys.2021.655695"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04001-4"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2020.07.015"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.2147\/CIA.S309082"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8808677"},{"issue":"126","key":"e_1_2_11_24_1","article-title":"Novel object recognition test for the investigation of learning and memory in mice","author":"Lueptow L. M.","year":"2017","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.748637"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2FO00569G"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aex354"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-017-0408-1"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2018.00752"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-160884"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2015.10.011"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118473"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00040-6"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-018-0174-9"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.12938\/bmfh.2021-056"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101871"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.jin2103079"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-019-00532-y"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.25065","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.25065","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.25065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:15:16Z","timestamp":1759943716000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.25065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,12]]},"references-count":37,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/ar.25065"],"URL":"https:\/\/doi.org\/10.1002\/ar.25065","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,12]]},"assertion":[{"value":"2022-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}