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However, there are few researches on EA for PD constipation. Autophagy is thought to be involved in the mechanistic process of EA in the central nervous system (CNS) intervention in Parkinson's pathology. However, whether it has the same effect on the enteric nervous system (ENS) has not been elucidated. Therefore, we investigated whether EA at <jats:italic>Tianshu<\/jats:italic> (ST25) acupoint promotes the clearance of \u03b1\u2010Syn and damaged mitochondria aggregated in the ENS in a model of rotenone\u2010induced PD constipation. This study evaluated constipation symptoms by stool characteristics, excretion volume, and water content, and the expression levels of colonic ATG5, LC3II, and Parkin were detected by Western Blot (WB) and Real\u2010Time Quantitative PCR (RT\u2010qPCR). The relationship between the location of \u03b1\u2010Syn and Parkin in the colonic ENS was observed by immunofluorescence (IF). The results showed that EA intervention significantly relieved the symptoms of rotenone\u2010induced constipation in PD rats, reversed the rotenone\u2010induced down\u2010regulation of colonic ATG5, LC3II, and Parkin expression, and the positional relationship between colonic \u03b1\u2010Syn and Parkin proved to be highly correlated. It is suggested that EA might be helpful in treating PD constipation by modulating Parkin\u2010induced mitochondrial autophagy.<\/jats:p>","DOI":"10.1002\/ar.25148","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T08:05:47Z","timestamp":1674115547000},"page":"3214-3228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The effect of electroacupuncture at <scp>ST25<\/scp> on Parkinson's disease constipation through regulation of autophagy in the enteric nervous system"],"prefix":"10.1002","volume":"306","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0402-1012","authenticated-orcid":false,"given":"Li\u2010Zhe\u2010Xiong","family":"Song","sequence":"first","affiliation":[{"name":"Key Laboratory of Acupuncture and Medicine Research of Ministry of Education Nanjing University of Chinese Medicine  Nanjing 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