{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:49:33Z","timestamp":1761061773788,"version":"build-2065373602"},"reference-count":67,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T00:00:00Z","timestamp":1674432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tight junctions (TJ) are the anatomical component of blood\u2010testis (BTB) and blood\u2010epididymis (BEB) barriers and contain many proteins, including claudins. The presence of claudins in domestic cat testis and epididymis has not been previously described. This study aimed to determine whether claudin\u20101 and claudin\u20105 participate in the structure of BTB and BEB and whether their amounts differ between the testis and epididymal segments of adult cats, using Western blotting (WB) and immunohistochemistry. WB results demonstrated that claudin\u20101 was significantly lower in the testis than in all epididymal segments and higher in the corpus epididymis than in the cauda, while claudin\u20105 in the testis was significantly lower than in the caput and corpus. Claudin\u20101 was absent at the Sertoli\u2010Sertoli junctions, while claudin\u20105 was detected at the level of the BTB during stages I and VIII. Both claudins were observed in the pachytene spermatocytes and the developing acrosome of the round and elongating spermatids. Claudin\u20105 was also detected in the cytoplasm of some spermatogonia, Sertoli cells, and late spermatid acrosome. In the epididymal segments, both claudins were localized to the area of the tight junctions and along the entire length of the lateral plasma membranes of adjacent principal cells and between principal and basal cells. These results may indicate that in the domestic cat, claudin\u20101 and \u20105 participate as both tight junction proteins and adhesion molecules in the BEB's structure, claudin 5 is a component of the BTB, and both proteins may be involved in postmeiotic germ cell development, especially acrosome development.<\/jats:p>","DOI":"10.1002\/ar.25165","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T08:49:21Z","timestamp":1674463761000},"page":"2153-2169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The abundance and localization of claudin\u20101 and \u20105 in the adult tomcats (<i>Felis catus<\/i>) testis, tubules rectus, rete testis, efferent ductules, and epididymis"],"prefix":"10.1002","volume":"306","author":[{"given":"Narin","family":"Liman","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04439.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1101-0"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD13301"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.45.151"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.11.028"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2020.1779526"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21200"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00038.2002"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2007.00308.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.059246"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.062760"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.010652"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200110122"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13360"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182106"},{"key":"#cr-split#-e_1_2_10_17_1.1","unstructured":"Godoy Morales H. S. Lozano S\u00e1nchez J. M. Sandoval Traves\u00ed A. Merchand \u00c1lvarez P. B. Romo Aguirre C. Mej\u00eda Biology R. M. et al. (2017).Claudin family expression in testis"},{"key":"#cr-split#-e_1_2_10_17_1.2","unstructured":"a dynamic membrane behavior for sperm formation in patients with infertility. American Society of Andrology42nd Annual Conference \"New Concepts and Perspectives in Male Reproductive Health\"April 21-25 2017 The Hilton Miami Downtown Miami FL."},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20467"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4161\/21565562.2014.979619"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.2.7975"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.68.213"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2012"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2012.02.007"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/01485010390204913"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2013-1878"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-0679-9_4"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/83.s1.109"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1230517"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20597"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.057323"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.039719"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007950200017"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.134965"},{"key":"e_1_2_10_34_1","first-page":"1","article-title":"A revised taxonomy of the Felidae. The final report of the cat classification task force of the IUCN\/SSC cat specialist group","volume":"11","author":"Kitchener A. C.","year":"2017","journal-title":"Cat News"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2016.17.4.445"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.02.001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.07.030"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2019.106247"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12122"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00972"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.078907"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD12259"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506084102"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.01.028"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.087452"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.3.579"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.1.185"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2010.0025"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.077040"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0022"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2014-1101"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1978.tb00606.x"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.110.010611"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.12750\/JARB.34.3.190"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.03.001"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.05.009"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2605.2000.00210.x"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.027284"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.10.020"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.188"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13346"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160349"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0572"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.02.004"},{"key":"e_1_2_10_65_1","first-page":"181","article-title":"Sperm quiescence in cauda epididymis: A mini review","volume":"3","author":"Verma R. J.","year":"2001","journal-title":"Asian Journal of Andrology"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0264.2004.00564.x"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-070342"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.25165","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.25165","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.25165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:55:24Z","timestamp":1759974924000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.25165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,23]]},"references-count":67,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/ar.25165"],"URL":"https:\/\/doi.org\/10.1002\/ar.25165","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"type":"print","value":"1932-8486"},{"type":"electronic","value":"1932-8494"}],"subject":[],"published":{"date-parts":[[2023,1,23]]},"assertion":[{"value":"2022-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}