{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:08:26Z","timestamp":1790870906258,"version":"4.1.0"},"reference-count":127,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T00:00:00Z","timestamp":1694563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008920","name":"University of Saskatchewan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008920","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Industrialization influenced several facets of lifestyle, including softer nutrient\u2010poor diets that contributed to vitamin D deficiency in post\u2010industrzialized populations, with concomitantly increased dental problems. Here we simulated a post\u2010industrialized diet in a mouse model to test the effects of diet texture and vitamin D level on mandible and third molar (M3) forms. Mice were raised on a soft diet with vitamin D (VitD) or without it (NoD), or on a hard diet with vitamin D. We hypothesized that a VitD\/hard diet is optimal for normal mandible and tooth root form, as well as for timely M3 initiation. Subsets of adult NoD\/soft and VitD\/soft groups were bred to produce embryos that were micro\u2010computed tomography (\u03bcCT) scanned to stage M3 development. M3 stage did not differ between embryos from mothers fed VitD and NoD diets, indicating that vitamin D does not affect timing of M3 onset. Sacrificed adult mice were \u03bcCT\u2010scanned, their mandibles 3D\u2010landmarked and M3 roots were measured. Principal component (PC) analysis described the largest proportion of mandible shape variance (PC1, 30.1%) related to diet texture, and nominal shape variance (PC2, 13.8%) related to vitamin D. Mice fed a soft diet had shorter, relatively narrower, and somewhat differently shaped mandibles that recapitulated findings in human populations. ANOVA and other multivariate tests found significantly wider M3 roots and larger root canals in mice fed a soft diet, with vitamin D having little effect. Altogether our experiments using a mouse model contribute new insights about how a post\u2010industrial diet may influence human craniodental variation.<\/jats:p>","DOI":"10.1002\/ar.25315","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T10:07:03Z","timestamp":1694599623000},"page":"611-632","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Food texture and vitamin <scp>D<\/scp> influence mouse mandible form and molar roots"],"prefix":"10.1002","volume":"307","author":[{"given":"Elsa M.","family":"Van Ankum","sequence":"first","affiliation":[{"name":"Department of Anatomy, Physiology &amp; Pharmacology University of Saskatchewan  Saskatoon Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kadin B.","family":"Majcher","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of Saskatchewan  Saskatoon Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan T.","family":"Dolovich","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of Saskatchewan  Saskatoon Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Johnston","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of Saskatchewan  Saskatoon Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kennedy P.","family":"Flegel","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology &amp; Pharmacology University of Saskatchewan  Saskatoon Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1147-364X","authenticated-orcid":false,"given":"Julia C.","family":"Boughner","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology &amp; Pharmacology University of Saskatchewan  Saskatoon Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1537-5110(02)00264-7"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-14-85"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199604000-00013"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12059"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330690409"},{"key":"e_1_2_9_7_1","first-page":"61","article-title":"Effects of dietary consistency on craniofacial and occlusal development in the rat","volume":"51","author":"Beecher R. M.","year":"1981","journal-title":"The Angle Orthodontist"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0189707"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(87)80010-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1016"},{"key":"e_1_2_9_11_1","first-page":"153","article-title":"Ultrastructural changes of a tooth root in young rats fed a low calcium and vitamin D\u2010deficient diet","volume":"42","author":"Bielaczyc A.","year":"1997","journal-title":"Roczniki Akademii Medycznej w Bialymstoku"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118916148.ch4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017535728844"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.12.012"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2009.00707.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchb.2017.08.002"},{"key":"e_1_2_9_17_1","article-title":"Maintaining perspective on third molar extraction","volume":"79","author":"Boughner J. C.","year":"2013","journal-title":"Journal of the Canadian Dental Association"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.22734"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2019.105563"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2008-0004"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-101020-4.00002-1"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1115\/1.3636535"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.606947"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201800140"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00718"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702884104"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/jhev.1993.1048"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-012-9633-2"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjy073"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jas.2016.06.006"},{"key":"e_1_2_9_31_1","volume-title":"Statistical shape analysis","author":"Dryden I. L.","year":"1998"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2013-1009"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118704868.ch20"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01003.2005"},{"key":"e_1_2_9_35_1","volume":"219","author":"Frost, H. M","journal-title":"The Anatomical Record"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12449"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1991.tb01825.x"},{"key":"e_1_2_9_38_1","volume":"2","author":"Gowland, R., Caffell, A., Newman, S., Levene, A., & Holst, M","journal-title":"Bioarchaeology International"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2012.12.001"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1500323"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00783"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0909-8836.2003.00080.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/25.2.315"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330720304"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40463-3"},{"key":"e_1_2_9_46_1","volume-title":"Dietary reference intakes for calcium and vitamin D","author":"Institute of Medicine, Food and Nutrition Board","year":"2010"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpp.2018.01.005"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.085084"},{"key":"e_1_2_9_49_1","volume":"68","author":"Jilka, R.L","journal-title":"Journal of Gerontology Series A: Biological Sciences and Medical Sciences"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420-9101.2012.02612.x"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-4603.1974.tb01441.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2005.12.008"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1702586114"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510362970"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6251"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.patbio.2004.12.005"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2010.02924.x"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/jdb9010008"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-4533(01)00038-8"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2004.09.025"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2004.03.116"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.2307\/j.ctvjnrtmh"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhevol.2004.03.005"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21127"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345211011041"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20674"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.111.2.269"},{"key":"e_1_2_9_68_1","first-page":"131","article-title":"Postnatal development of the craniofacial skeleton in male C57BL\/6J mice","volume":"55","author":"Maga A. M.","year":"2016","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016357.2016.1209240"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2019.06.010"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.3390\/reports3010001"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01685"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchb.2014.12.001"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.22560"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0469.2006.00390.x"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1093\/iob\/obab030"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1928"},{"key":"e_1_2_9_78_1","unstructured":"National Institutes of Health Office of Dietary Supplements. (2022).Vitamin D fact sheet for health professionals.https:\/\/ods.od.nih.gov\/factsheets\/VitaminD-HealthProfessional\/#en1"},{"key":"e_1_2_9_79_1","volume-title":"Nutrient requirements of laboratory animals","author":"Nutrition NRCUSoLA","year":"1995"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.04.033"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1998.19320251.x"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910308200415"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.10084"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.7208\/chicago\/9780226705965.001.0001"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS2003297"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/ede.12026"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1111\/ede.12261"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117301"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45279-9"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25309-8"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cji028"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2013.10.001"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1345"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.11.1939"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-12-141"},{"key":"e_1_2_9_96_1","volume":"30","author":"Renaud, S., & Michaux, J. R","journal-title":"Journal of Biogeography"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200002)217:2<137::AID-DVDY1>3.0.CO;2-N"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sodo.2006.08.003"},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(78)90209-1"},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.2307\/2992207"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02733"},{"key":"e_1_2_9_102_1","volume-title":"The principles of humane experimental technique","author":"Russell W. M. S.","year":"1959"},{"key":"e_1_2_9_103_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-10-18"},{"key":"e_1_2_9_104_1","first-page":"4099","article-title":"Teaching an old jaw new tricks: Diet\u2010induced plasticity in a model organism from weaning to adulthood","volume":"15","author":"Scott J. E.","year":"2014","journal-title":"The Journal of Experimental Biology"},{"key":"e_1_2_9_105_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12103005"},{"key":"e_1_2_9_106_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101471"},{"key":"e_1_2_9_107_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-24293-3"},{"key":"e_1_2_9_108_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(83)90030-4"},{"key":"e_1_2_9_109_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2018.01.009"},{"key":"e_1_2_9_110_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasrep.2018.07.019"},{"key":"e_1_2_9_111_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10764-005-9000-5"},{"key":"e_1_2_9_112_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88418-4"},{"key":"e_1_2_9_113_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12384"},{"key":"e_1_2_9_114_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23358"},{"key":"e_1_2_9_115_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-018-2766-6"},{"key":"e_1_2_9_116_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsfs.2020.0087"},{"key":"e_1_2_9_117_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00273"},{"key":"e_1_2_9_118_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1113050108"},{"key":"e_1_2_9_119_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.22556"},{"key":"e_1_2_9_120_1","doi-asserted-by":"publisher","DOI":"10.5744\/bi.2018.1020"},{"key":"e_1_2_9_121_1","doi-asserted-by":"publisher","DOI":"10.1080\/03014223.2017.1411955"},{"key":"e_1_2_9_122_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-802928-2.00043-6"},{"key":"e_1_2_9_123_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11692-009-9056-9"},{"key":"e_1_2_9_124_1","unstructured":"Yu C.(2019).Positive secular change in dental maturation over a 30\u2010year period. The University of British Columbia Theses and Dissertations.https:\/\/doi.org\/10.14288\/1.0379918"},{"key":"e_1_2_9_125_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1420-9101.2003.00568.x"},{"key":"e_1_2_9_126_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2006-0015"},{"key":"e_1_2_9_127_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.01.002"},{"key":"e_1_2_9_128_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.06.010"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.25315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T19:32:09Z","timestamp":1759951929000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.25315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,13]]},"references-count":127,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/ar.25315"],"URL":"https:\/\/doi.org\/10.1002\/ar.25315","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,13]]},"assertion":[{"value":"2023-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}